We narrowed to 6,639 results for: WUS;

Showing: 5641 - 5680 of 6639 results

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PB-Neo-TetO-Cdk13_Untagged

Plasmid

#127344

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

TetO Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8/GW_TOPO_Cdk13_Untagged

Plasmid

#127342

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AP-SpyCatcher

Plasmid

#223636

Purpose

Protein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

His10, AP fragment of split APEX2 tag

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6p1_RBFOX2_RRM

Plasmid

#224293

Purpose

GST-RBFOX2(RRM domain) purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 RNA-recognition motif (RBFOX2 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 74-167

Promoter

tac promoter

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-effector-ncRNA-rrt-His8

Plasmid

#194089

Purpose

Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 effector-ncRNA-rrt

Tags

His8

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-1

Plasmid

#210139

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCYFIP1-1

Plasmid

#210137

Purpose

knock out CYFIP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgABI2-2

Plasmid

#210140

Purpose

knock out ABI2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Abl interactor 2 (ABI2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-2

Plasmid

#210136

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgWAVE2-1

Plasmid

#210135

Purpose

knock out WAVE2 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Actin-binding protein WASF2 (WASF2 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgHSPC300-1

Plasmid

#210141

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgHSPC300-2

Plasmid

#210142

Purpose

knock out HSPC300 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Protein BRICK1 (BRK1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_8xTEAD_luc2

Plasmid

#173005

Purpose

firefly luciferase reporter vector with 8x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

8xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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anti-Calbindin scFv [L109/57] with sortase tag

Plasmid

#204421

Purpose

Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Calbindin (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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anti-GFAP R416WT scFv [N206B/9] with sortase tag

Plasmid

#204420

Purpose

Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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anti-Parvalbumin scFv [L114/81] with sortase tag

Plasmid

#204423

Purpose

Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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anti-Kv1.2 scFv [K14/16] with sortase tag

Plasmid

#204425

Purpose

Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

Depositor

Jeff Lichtman

Article

Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:

Insert

Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-79

Plasmid

#197397

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-79

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-79 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse ZNG1-METAP1 fusion

Plasmid

#197398

Purpose

Expresses a SUMO-tagged fusion of ZNG1, residues 10-30 and mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1-METAP1 (Cbwd1, Metap1 Mouse)

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-59

Plasmid

#197396

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-59 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

Plasmid

#195502

Purpose

Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

HA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

Plasmid

#195495

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgT-REX17_EF1a-Puro-T2A-BFP

Plasmid

#195501

Purpose

Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

BFP, Puro, and T2A

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP-e2a-SOX2-p2a-KLF4

Plasmid

#193358

Purpose

VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP-e2a-SOX2-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pVPSBA01-SARS-CoV2-nsp1

Plasmid

#192171

Purpose

express SARS2-NSP1

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVPSBA01-SARS-CoV2-nsp1 mutant4

Plasmid

#192174

Purpose

express SARS2-NSP1-mut4

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-mut4 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVPSBA01-SARS-CoV2-nsp1-truncated

Plasmid

#192172

Purpose

express SARS2-NSP1-trunc

Depositor

Sidi Chen

Article

Yuan et al Mol Cell. 2020 Dec 17;80(6):1055-1066.e6

Insert

express SARS2-NSP1-trunc (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-mRuby2

Plasmid

#176550

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-mADAR2-short

Plasmid

#175588

Purpose

mammalian expression of mouse short Adar2, blasticidine selection

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

Short Mouse Adar2

Tags

Flag

Expression

Mammalian

Promoter

cmv

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-mWasabi

Plasmid

#176552

Purpose

Expression of mWasabi for auxotrophic selection in the absence of leucine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

mWasabi

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-eCFP

Plasmid

#176558

Purpose

Expression of eCFP for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pH-TagRFP657

Plasmid

#176560

Purpose

Expression of TagRFP657 for auxotrophic selection in the absence of lysine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

TagRFP657

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459_GRHL1-exon5

Plasmid

#177763

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX459_GRHL1-exon8

Plasmid

#177764

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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