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We narrowed to 12,964 results for: BASE

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Showing: 7051 - 7100 of 12964 results

  4. pET-30a(+)-C-SH2+IA

    Plasmid
    #111418
    Purpose
    express murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Protein Sci. 2018 Jul 27. doi: 10.1002/p
    Insert
    murine Syk (C)SH2 domain plus interdomain A (Phe 119 to Gln 264), without any tag (Syk Mouse)
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111418-plasmid-map-sequence-id-215713

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  6. pMflT-o3

    Plasmid
    #101311
    Purpose
    Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Insert
    oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101311-plasmid-map-sequence-id-198141

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  7. PatoM-OG241

    Plasmid
    #72842
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoM
    Expression
    Bacterial
    Promoter
    PatoM
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72842-plasmid-map-sequence-id-133606

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  9. pUAST-HA, Pbl-A DH domain

    Plasmid
    #69794
    Purpose
    Pebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble DH domain
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA-Tag
    Expression
    Insect
    Mutation
    Contains only the Pebble DH domain
    Promoter
    hsp70
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Promoter
    none
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  11. sgFFL_7x_Bulge

    Plasmid
    #59894
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has seven bulge targets (fully comple…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59894-plasmid-map-sequence-id-108252

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  12. pLS-mP-Luc

    Plasmid
    #106253
    Purpose
    Luciferase lentivirus based enhancer assay vector
    Depositor
    Nadav Ahituv
    Insert
    Luciferase
    Use
    Lentiviral and Luciferase
    Promoter
    Derived from pGL4.23
    Available Since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106253-plasmid-map-sequence-id-208513

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  13. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  14. Lentiviral nontargeting shRNA GFP

    Plasmid
    #155285
    Purpose
    Lentiviral expression of nontargeting shRNA, GFP expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155285-plasmid-map-sequence-id-311904

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  15. MZB184

    Plasmid
    #228488
    Purpose
    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    10xHis-SUMO(Smt3)
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228488-plasmid-map-sequence-id-458447

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  16. MZB223

    Plasmid
    #228491
    Purpose
    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    8xHis
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228491-plasmid-map-sequence-id-455392

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  17. pGGD_RT_biChromium

    Plasmid
    #136974
    Purpose
    Goldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptide
    Depositor
    Klaus Theres
    Insert
    P2A sequence
    Expression
    Bacterial
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136974-plasmid-map-sequence-id-270595

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  18. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Expression
    Mammalian
    Available Since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  19. pCfb12620

    Plasmid
    #219928
    Purpose
    PrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TPI
    Expression
    Yeast
    Available Since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    219928-plasmid-map-sequence-id-487419

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  20. Lentiviral nontargeting shRNA RFP i670

    Plasmid
    #155287
    Purpose
    Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155287-plasmid-map-sequence-id-310003

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  21. pMIMY

    Plasmid
    #191954
    Purpose
    A pMSCV-based backbone where the MCS has been substituted with a MCS-IRES-mitoYFP for expression of a gene of interest and a mitochondrially targeted YFP
    Depositor
    Luca Scorrano
    Type
    Empty backbone
    Use
    Retroviral
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191954-plasmid-map-sequence-id-379817

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  22. Dox nontargeting shRNA mKate

    Plasmid
    #155292
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155292-plasmid-map-sequence-id-310436

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  23. Dox nontargeting shRNA GFP

    Plasmid
    #155290
    Purpose
    Lentivirus for doxycycline inducible expression of nontargeting shRNA, GFP marker, based on Addgene 11652
    Depositor
    Martin Carroll
    Insert
    Nontargeting shRNA
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    155290-plasmid-map-sequence-id-475763

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  24. pMH_AtCK2alpha3_1-331_HC

    Plasmid
    #238512
    Purpose
    Bacterial expression of A. thaliana CK2alpha3 1-331_HC
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha3 (AT2G23080 Mustard Weed)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238512-plasmid-map-sequence-id-477131

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  25. pCfb12567

    Plasmid
    #219927
    Purpose
    PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter CYC
    Expression
    Yeast
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219927-plasmid-map-sequence-id-460324

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  26. pCfb12566

    Plasmid
    #219926
    Purpose
    PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter DGA
    Expression
    Yeast
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    219926-plasmid-map-sequence-id-460318

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  27. pCfb12622

    Plasmid
    #219929
    Purpose
    PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter ACT
    Expression
    Yeast
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219929-plasmid-map-sequence-id-436186

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  28. pCfb12562

    Plasmid
    #219923
    Purpose
    PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter GPD
    Expression
    Yeast
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219923-plasmid-map-sequence-id-460320

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  29. pCfb13186

    Plasmid
    #219924
    Purpose
    PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter TEF
    Expression
    Yeast
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219924-plasmid-map-sequence-id-436038

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  30. pCfb12561

    Plasmid
    #219922
    Purpose
    PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter PGM
    Expression
    Yeast
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219922-plasmid-map-sequence-id-435639

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  31. pCfb12565

    Plasmid
    #219925
    Purpose
    PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI
    Depositor
    Irina Borodina
    Insert
    Promoter FBA
    Expression
    Yeast
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219925-plasmid-map-sequence-id-435638

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  32. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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  33. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  36. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Article
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  40. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  43. qTAG-N-Solo-mStayGold-CENPA

    Plasmid
    #227283
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227283-plasmid-map-sequence-id-451297

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  46. pLJM1-EGFP-GRAM-W

    Plasmid
    #211704
    Purpose
    Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    EGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211704-plasmid-map-sequence-id-419216

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  49. EGFP-GRAM-W

    Plasmid
    #211701
    Purpose
    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211701-plasmid-map-sequence-id-419225

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  50. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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Showing: 7051 - 7100 of 12964 results