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Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPGN
Plasmid#199232PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertEpithelial mitogen isoform 6 (EPGN Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn deltaSH3
Plasmid#16034DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDelta SH3: deletion of amino acids 76-141PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn SH2:R176E
Plasmid#16035DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationSH2 point mutant: R176EPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-Lact-C2
Plasmid#109553PurposeMultisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertthe C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8).
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_eZ3_Gal4VP64
Plasmid#169917PurposeExpression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.DepositorInsertantiCD19-Notch3-GL4VP64
UseLentiviralTagsExpressionMammalianMutationAdditional EGF repeat between the extracellular d…PromoterPGKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
psd44-Lactadherin46
Plasmid#46830Purposeexpression of human lactadherin in mammalian cellsDepositorInsertMFGE8 (MFGE8 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
SRpHi4
Plasmid#102544PurposeBacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purificationDepositorInsertEYFP-EGF
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 mCit mCer
Plasmid#83563PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPRKCA (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-LactC2
Plasmid#109499PurposeMuscle specific zebrafish expression of mKate2 fused to the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)DepositorInsertactc1b-mKate2-LactC2
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf3
Plasmid#12691PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
5HRE/GFP
Plasmid#46926Purposehypoxia-responsive enhanced green fluorescent protein (EGFP)-based systemDepositorInsert5X HRE of VEGF (VEGFA Human)
UseTagsd2EGFPExpressionMammalianMutationfive copies of a 35-bp fragment from the hypoxia-…PromoterEF-1α promoterAvailable sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLT1-KDR
Plasmid#86034Purposechimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2DepositorUseTagsHA and t-GFPExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5HRE-GFP PuroR
Plasmid#128958PurposeLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent proteinDepositorInsert5X HRE of VEGF (VEGFA Human)
UseLentiviralTagsd2EGFPExpressionMammalianMutationPromoterminimal CMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5HRE-GFP NeoR
Plasmid#128960PurposeLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent proteinDepositorInsert5X HRE of VEGF (VEGFA Human)
UseLentiviralTagsd2EGFPExpressionMammalianMutationPromoterminimal CMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CIRC#del
Plasmid#186344PurposeContains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRC
Plasmid#186343PurposeContains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)DepositorInsertVEGFA (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
psd44-iGFP-MFGE8-long
Plasmid#46847Purposeexpression of MFGE8 in mammalian cellsDepositorInsertMFGE8 (Mfge8 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pWPT-mCherry
Plasmid#190605PurposeObtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherryDepositorInsertsmEGFP
mCherry
UseLentiviralTagsExpressionMutationEGFP Kozak sequence has been replaced with a stre…PromoterEIF1-shortAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS1253
Plasmid#29129PurposeExpression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.DepositorInsertPle108 (HBEGF Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorInsertneuropilin 1 (NRP1 Human)
UseTagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEMS1252
Plasmid#29128PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle107 (HBEGF Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1254
Plasmid#29130PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle109 (HBEGF Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-1-mtGFP
Plasmid#138981PurposeNeuron-specific expression of membrane targeted EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationEGFP (added membrane targeting domain from GAP43).PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorInsertVEGFA (Vegfa Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
hFLT1 delta CTD
Plasmid#86036Purposehuman VEGFR1 with deletion of the entire intracellular domainDepositorInserthuman Flt1 delta CTD (FLT1 Human)
UseTagsFLAG and HAExpressionMammalianMutationdeta CTDPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 Y1333F
Plasmid#86037Purposehuman VEGFR1 with Y to F mutation at position 1333DepositorInserthuman Flt1 Y1333F (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationY1333F; HA tag is inserted downstream of signal …PromoterCMVAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorInsertPE7-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1-SpRY (pBM1571)
Plasmid#170105PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFPDepositorInsertrAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationR33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…PromoterCMVAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1m
Plasmid#120955PurposeMoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)DepositorInsertdeGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_221
Plasmid#169142PurposeConstitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.DepositorInsertEGFP , EGFP sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 Ultra
Plasmid#106172Purposelentiviral CMV driven eGFP-P2A-T2A, Blasticidin selectableDepositorInserteGFP
UseLentiviralTagsT2A, P2AExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-m-Flt4
Plasmid#119232PurposePlasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.DepositorInsertVegfr3 (Flt4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pEMS1255
Plasmid#29131PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle110 (HBEGF Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pBB14
Plasmid#18657DepositorInsertUs9-EGFP gene fusion
UseTagsEGFPExpressionMammalianMutationFirst 106 amino acids of Us9 are intact. The stop…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMEclecl14aP1gfp
Plasmid#90123PurposeGateway Mid-entry clone containing promoter sequence specific for clec14a driving expression of eGFPDepositorInsertclec14aP1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEnrp1bP1gfp
Plasmid#90129PurposeGateway Mid-entry clone containing promoter sequence specific for nrpb1b driving expression of eGFPDepositorInsertnrp1bP1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEmafbbP1gfp
Plasmid#90128PurposeGateway Mid-entry clone containing promoter sequence specific for maffbb driving expression of eGFPDepositorInsertmaffbbP1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only