We narrowed to 17,082 results for: EMB

1. 10_T7-SP-CD8tm-mScarlet3

   Plasmid

   #225937

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   SP-CD8tm-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hnRNPA2_LC_nocharge

   Plasmid

   #139118

   Purpose

   express hnRNPA2 LC with no charged residues

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Expression

   Bacterial

   Mutation

   all charged residues are changed to S/Q except fo…

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Stargazin-dCherry-FRB

   Plasmid

   #172444

   Purpose

   Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Cacng2 (Cacng2 Mouse)
   

   Tags

   dCherry-HA-FRB

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. USPL1cat-pETM30

   Plasmid

   #85768

   Purpose

   bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   T7-lac

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

   Plasmid

   #79259

   Purpose

   Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   DmeIF4G (eIF4G1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   V5-His6 and lambdaN-HA

   Promoter

   Ac5 promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RepairTemplate_Smc4-mAID-HaloTAG

   Plasmid

   #217658

   Purpose

   Repair template to endogenously tag SMC4 with mAID-Halo Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   SMC4-mAID-HaloTag (SMC4 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. USPL1cat-pEYFP_C1

   Plasmid

   #85766

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKK-HA-TEV

   Plasmid

   #105769

   Purpose

   Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   HA-TEV

   Expression

   Mammalian

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKK-TEV-HA

   Plasmid

   #105785

   Purpose

   Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-HA

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-PM-InPAkt

    Plasmid

    #181915

    Purpose

    Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    pm-InPAkt
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Contains the following mutations with respect to …

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NET50 pmRFP-N2 (489)

    Plasmid

    #61983

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET50 (DHRS7 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. NET97 pmRFP-N2 (641)

    Plasmid

    #61994

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    AADACL1 (NCEH1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pKK-TEV-FLAG

    Plasmid

    #105783

    Purpose

    Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-PBD H538A/K540M

    Plasmid

    #60879

    Purpose

    Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA 365-653 H538A/K540M mutant

    Depositor

    Trina Schroer

    Article

    Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

    Insert

    Polo-like kinase 1 (PLK1 Human, BC01486)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H538A/K540M

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

15. NET37 pmRFP-N2 (450)

    Plasmid

    #61980

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET37 (KIAA1161 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pPBpuro-mNG-eDHFR(69K6)-Tiam1

    Plasmid

    #172102

    Purpose

    Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FB003

    Plasmid

    #119677

    Purpose

    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC:hph:Ttub
    

    Use

    Synthetic Biology

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAPBPL pEGFP-C3 (1765)

    Plasmid

    #62055

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-PTEN-deltaPDZ

    Plasmid

    #110182

    Purpose

    Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

    Depositor

    Jose Esteban

    Article

    Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

    Insert

    PTEN (Pten Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of last 4 amino acids

    Promoter

    CMV

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAV10.F6.loxP

    Plasmid

    #63201

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge