We narrowed to 18,386 results for: Met

1. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_flGalT-SBP-EGFP

   Plasmid

   #65272

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-I-PhoCl-HCVp

   Plasmid

   #87697

   Purpose

   Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
   

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-neo FlucSM EGFP

   Plasmid

   #90171

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Expression

   Mammalian

   Mutation

   R188Q in FLuc

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos4b-C1

   Plasmid

   #54812

   Purpose

   Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson, Loren Looger

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mEos4b

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2

   Plasmid

   #87693

   Purpose

   Express ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
   

   Expression

   Mammalian

   Available Since

   May 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-KDEL_flGalT-SBP-mCherry

   Plasmid

   #65273

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-smURFP-IRES-eGFP

   Plasmid

   #80343

   Purpose

   Expresses smURFP and eGFP in mammalian cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   eGFP
   

   Expression

   Mammalian

   Promoter

   CMV and IRES

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NES-PhoCl-mCherry

    Plasmid

    #87690

    Purpose

    Express NES-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NES-PhoCl-mCherry
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA_tdtomato_reporter_1_SA-2x

    Plasmid

    #79369

    Purpose

    Assays activity of transcriptional activators fused to SA Cas9

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    tdtomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Clover-Geminin(1-110)

    Plasmid

    #83915

    Purpose

    Fluorescent probe for M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5-SUV39H2-FL

    Plasmid

    #89135

    Purpose

    mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    SUV39H2 (SUV39H2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-KDEL_SBP-mCherry-Ecadherin

    Plasmid

    #65287

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Arp2/3_pBIG2abcd

    Plasmid

    #173683

    Purpose

    Insect expression of the 7-subunit Human Arp2/3 complex

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Inserts

    Arp2
    Arp3
    ArpC2
    ArpC3-FLAG
    ArpC4
    ArpC5
    ArpC1
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUBC_HA_2xPP7-VP64

    Plasmid

    #68418

    Purpose

    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    PP7(deltaFG)
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    human UbC

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCry2PHR-mCh-LRP6c

    Plasmid

    #42960

    Purpose

    Expresses optogenetically controlled LRP6 (C-terminal domain)

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CASI-Cas9C

    Plasmid

    #80932

    Purpose

    Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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