We narrowed to 4,430 results for: TIL;

1. pCAGGS-SspB-mScarlet-I-MYPT169

   Plasmid

   #178522

   Purpose

   Expresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   SspB-mScarlet-I-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

   Plasmid

   #71719

   Purpose

   Expressing a GFP-tagged degron in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   auxin-responsive protein IAA17 (AXR3 Mustard Weed)
   

   Tags

   EmGFP

   Expression

   Worm

   Mutation

   minimal degron sequence (71-114aa) with start cod…

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS2E

   Plasmid

   #55169

   Purpose

   Empty vector, integration at lacA, ampr, mlsr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCS6-YFP-SMASh(AI)

   Plasmid

   #111500

   Purpose

   Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   YFP-SMASh(AI)
   

   Tags

   HA epitope tag and SMASh tag

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries V36M, T54A, and S122…

   Promoter

   CMV

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NO lux Sensor

   Plasmid

   #199790

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   NO lux Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-gst-xr

   Plasmid

   #214761

   Purpose

   Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

   Depositor

   Pimchai Chaiyen

   Article

   Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

   Insert

   glutathione S-transferases fused with xylose reductase
   

   Tags

   Glutathione S-transferases

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFlp-Gm

   Plasmid

   #222335

   Purpose

   Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Promoter

   cl, flp, and sacB

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b_RanT24N

    Plasmid

    #186271

    Purpose

    Expression vector of His-RanT24N in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Tags

    His tagged and His-tagged at N terminal

    Expression

    Bacterial

    Mutation

    Changed T24 into N

    Promoter

    T7 promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-GIT1

    Plasmid

    #15226

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L425V, E476K and L583P

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P41nmt1-GFP

    Plasmid

    #39290

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-kanMX6-P81nmt1-GFP

    Plasmid

    #39291

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Mutation

    -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TT lux sensor

    Plasmid

    #199792

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    TT lux Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMuLE ENTR CMV Renilla Luciferase L5-L2

    Plasmid

    #62186

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Renilla luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

    Plasmid

    #85041

    Purpose

    This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    loxP-stop-loxP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107320

    Purpose

    Expresses SpCas9 fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to SaCas9

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP - FLAG-APEX2- hs Lamin B1

    Plasmid

    #139442

    Purpose

    APEX2-Lamin B1 expression plasmid

    Depositor

    Yixian Zheng

    Article

    Tran et al bioRxiv 2020.02.05.935635

    Insert

    LMNB1 (LMNB1 Human)
    

    Tags

    3xGGGGS linker (between APEX2 and Lamin B1), APEX…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-mGold-Calnexin-N-14

    Plasmid

    #158004

    Purpose

    Visualization of ER in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Calnexin (CANX Human)
    

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-VTKS1

    Plasmid

    #170852

    Purpose

    AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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