We narrowed to 26,284 results for: STI

1. pRExT2A-NT-BmNG

   Plasmid

   #232204

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mNeonGreen

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRExT2A-NT-BmSc

   Plasmid

   #232205

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: BSD-3xTy-T2A-3xTy-mScarlet

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRExT2A-CT-BmSc

   Plasmid

   #232208

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLeo-A-a2-GEMS(PLCG)

   Plasmid

   #209114

   Purpose

   Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (PLCG)

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   A-a2-GEMS(PLCG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196075

   Purpose

   Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle.Cre

   Plasmid

   #192930

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pENTR4 Ta-SRO1 JN177

   Plasmid

   #192546

   Purpose

   pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 Ta-sro1 SR3

   Plasmid

   #192547

   Purpose

   pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENT-L1-Kozak-Ark-L2

   Plasmid

   #186362

   Purpose

   Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Ciona robusta Aurora kinase A
   

   Use

   Expression of a fluorescent centrosome marker

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a(+)_co_L-HicDH

    Plasmid

    #185453

    Purpose

    CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

    Depositor

    Wolfgang Kroutil

    Article

    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

    Insert

    L-HicDH from Lactobacillus confusus DSM 20196
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH1(GAP)

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1mcherry-NKD2EFMUT

    Plasmid

    #174333

    Purpose

    encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    NKD2132DFD134>AFA (substitutions of D132 and D…

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1A CbbL-

    Plasmid

    #162693

    Purpose

    p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    H. neapolitanus CbbLS and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    CbbL K194M (inactive rubisco)

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. zebrafish SLC2A2 (zfSLC2A2)

    Plasmid

    #65103

    Purpose

    Full length SLC2A2 cDNA (Danio rerio)

    Depositor

    Josep Planas

    Article

    Castillo et al Am J Physiol Regul Integr Comp Physiol.

    Insert

    SLC2A2 (slc2a2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

    Plasmid

    #83462

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, W4107A

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

    Plasmid

    #83460

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

    Plasmid

    #83463

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4169

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

    Plasmid

    #83459

    Purpose

    Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, S4144D

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

    Plasmid

    #83457

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303

    Promoter

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge