We narrowed to 32,062 results for: eng

1. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

   Plasmid

   #164514

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.

   Depositor

   Loren Looger

   Article

   Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHsa.NFKB:d2EGFP

   Plasmid

   #239990

   Purpose

   Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

   Depositor

   Raquel Espin-Palazon

   Insert

   Destabilized EGFP
   

   Promoter

   cfos minimal promoter

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKH3-TRIM28

   Plasmid

   #45569

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223249

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC3.1-caggs-Crimson-CAAX

   Plasmid

   #190041

   Purpose

   Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

   Depositor

   Michael Lin

   Article

   Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

   Insert

   Crimson
   

   Tags

   CAAX

   Expression

   Mammalian

   Promoter

   caggs

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM7

   Plasmid

   #201213

   Purpose

   Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

   Depositor

   Alex Nielsen

   Article

   Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

   Inserts

   sfGFP
   Spo0A right flank
   Spo0A left flank
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   F11V, T13P, N39D, A179A

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIRECT_23C

    Plasmid

    #91140

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5-Flag-HDAC3

    Plasmid

    #63676

    Purpose

    Mammalian expression of Flag tagged HDAC3

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

    Insert

    HDAC3 (Hdac3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 6xHsa.NFKB:d2mRFP1

    Plasmid

    #239991

    Purpose

    Destabilized red fluorescent protein (d2mRFP1) under transcriptional control of NF-kB activation

    Depositor

    Raquel Espin-Palazon

    Inserts

    mRFP1
    PEST
    

    Promoter

    cfos minimal promoter

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Puro CAG J591-scFV T-CAR

    Plasmid

    #171961

    Purpose

    Donor plasmid for J591 T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic J591-scFV CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-GRAB_rDA1h

    Plasmid

    #140557

    Purpose

    Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA sensor GRAB_rDA1h (high affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLVTHM-mSox2-3xflag

    Plasmid

    #206376

    Purpose

    Expresses 3xflag fused mouse SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)- e18

    Plasmid

    #131670

    Purpose

    Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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