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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-Ascl1-puro

Plasmid

#97329

Purpose

Generating lentiviruses to express Ascl1 and puromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Promoter

TET-ON

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAAVTREGCaMP6f

Plasmid

#97410

Purpose

Amplified expression of GCaMP6f in combination with TET driver

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

TRE3

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AAV.TBG.PI.Cre.rBG

Plasmid

#107787

Purpose

AAV vector expressing Cre from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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phU6-gRNA

Plasmid

#53188

Purpose

Expresses the S. pyogenes sgRNA from the human U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLX209-neo-active WRN

Plasmid

#125788

Purpose

constitutive, ectopic expression of human active WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-active WRN (WRN Human)

Tags

5x myc

Expression

Mammalian

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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