We narrowed to 12,703 results for: NUC
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Plasmid#174675PurposeExpresses 6xHis-MBP tagged PxCas12aDepositorArticleInserthuPxCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huPrCas12a
Plasmid#174674PurposeExpresses 6xHis-MBP tagged PrCas12aDepositorArticleInserthuPrCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH806_pJH_LldR_Pcon-sfGFP.1TAG
Plasmid#163737PurposeConstitutive expression of green fluorescent protein encoding one stop codonDepositorInsertlldr gfp.1TAG
ExpressionBacterialMutationgfp.N39TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH28_pBad_AraC_Para-sfGFP.3TAG
Plasmid#163723PurposeArabinose-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertaraC gfp.3TAG
ExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH626_pJH_LldR_PlldR-sfGFP.1TAG
Plasmid#163725PurposeLactate-inducible expression of green fluorescent protein encoding one amber codonDepositorInsertlldr gfp.1TAG
ExpressionBacterialMutationgfp.N39TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH627_pJH_LldR_PlldR-sfGFP.2TAG
Plasmid#163726PurposeLactate-inducible expression of green fluorescent protein encoding two amber codonsDepositorInsertlldr gfp.2TAG
ExpressionBacterialMutationgfp.N39TAG,Y135TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH721_pJH_LldR_PlldR-sfGFP.20TAG
Plasmid#163729PurposeLactate-inducible expression of green fluorescent protein encoding twenty amber codonsDepositorInsertlldr gfp.20TAG
ExpressionBacterialMutationgfp with all 20 leucine codons mutated to amberAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH727_pJH_LldR_PlldR-sfGFP.10TAG
Plasmid#163731PurposeLactate-inducible expression of green fluorescent protein encoding ten amber codonsDepositorInsertlldr gfp.10TAG
ExpressionBacterialMutationgfp with first 10 leucine codons mutated to amberAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH774_pJH_LldR_PlldR-sfGFP.1opal
Plasmid#163733PurposeLactate-inducible expression of green fluorescent protein encoding one opal codonDepositorInsertlldr gfp.1TGA
ExpressionBacterialMutationgfp with first leucine codon mutated to amberAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM_N1_400
Plasmid#170474PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O-, O1, Lambda t1 in modified lacZ fragment
ExpressionBacterialMutationdiminished hairpins near O1 and diminished latent…PromoterT7A1Available SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_035
Plasmid#109152PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFPDepositorInsertsmRFP
EGFP
UseSynthetic BiologyExpressionBacterial and MammalianPromoterCMV and pTetAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHY55-mouse U6-sgLMNA
Plasmid#164045PurposeU6-driven sgRNA targeting LMNADepositorInsertLMNA targeting sgRNA
UseCRISPRPromotermouse U6Available SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-2
Plasmid#164859PurposeConstitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-2
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-3
Plasmid#164860PurposeConstitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-3
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-3
Plasmid#164867PurposeConstitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-3
UseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-2
Plasmid#164866PurposeConstitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-2
UseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-ProSgH
Plasmid#163974PurposeHelper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_A34G_A35G
Plasmid#81232PurposeU6 telestem mutant (A34G/A35G) for in vivo studiesDepositorInsertU6 RNA with A34G/A35G mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (A34G/A35G)Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_G96C_A97C
Plasmid#81235PurposeU6 telestem mutant (G96C/A97C) for in vivo studiesDepositorInsertU6 RNA with G96C/A97C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (G96C/A97C)Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS314_U6_U100C_U101C
Plasmid#81236PurposeU6 telestem mutant (U100C/U101C) for in vivo studiesDepositorInsertU6 RNA with U100C_U101C mutation (snR6 Budding Yeast)
ExpressionYeastMutationU6 telestem mutant (G96C/A97C)Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-deltaKRMR-3xFLAG
Plasmid#130676PurposeExpresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 deltaKRMR (SMCHD1 Human)
Tags3xFLAGExpressionMammalianMutationK1987_R1990delPromoterCMVAvailable SinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
PTG
Plasmid#127198PurposeSingle template U6 promoter minimal size plasmidDepositorTypeEmpty backboneUseEmpty sgrna plasmidExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM587
Plasmid#133593PurposePart Plasmid for dCas9, Part 3b(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM586
Plasmid#133592PurposePart Plasmid for dCas9, Part 3(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD-NIa-Protease-TVMV
Plasmid#137109PurposeiFLinkC Linker cloningDepositorInsertNIa Protease TVMV
UseSynthetic BiologyAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS039
Plasmid#134233PurposeFor in vitro transcription of Miniata CENP-ADepositorInsertMiniata CENP-A n-terminus (nt 1-204, aa 1-68)
TagsGSTExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS077
Plasmid#134235PurposeBacterial expression of CENP-CDepositorInsertGST-CENP-C
TagsGSTExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH131
Plasmid#136550PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH130
Plasmid#136549PurposecrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)DepositorInsertcrRNA backbone for FnCas12a/CmsI nucleases, combine with OsU6-2 promoter module (pFH37)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only