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pPB-cT3G-cERP2-HOXA5

Plasmid

#222538

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HOXA5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

HOXA5 (HOXA5 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRT1

Plasmid

#222571

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRT1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRT1 (DMRT1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMRTC2

Plasmid

#222548

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMRTC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMRTC2 (DMRTC2 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOSIN

Plasmid

#222551

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOSIN

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOSIN (LOC388553 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DPPA3

Plasmid

#222578

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DPPA3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DPPA3 (DPPA3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-CTCFL

Plasmid

#222546

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of CTCFL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

CTCFL (CTCFL Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-3xFLAG-SMAD9-active

Plasmid

#222580

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD9

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMAD9 (SMAD9 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

S489E, S491E

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003_sgTFAP4 guide 1

Plasmid

#193450

Purpose

ko TFAP4 guide 1

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

Tfap4

Use

Lentiviral

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf

Plasmid

#182000

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_SKL

Plasmid

#182007

Purpose

CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf_SKL

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF2

Plasmid

#110653

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF1

Plasmid

#110652

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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UPATrap-VC-ORF0

Plasmid

#110651

Purpose

transposon containing BiFC VC cassette

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

Venus C-terminus cassette

Expression

Mammalian

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S10

Plasmid

#128594

Purpose

Expresses tagless S10 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S10 (rpsJ E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3-M129C

Plasmid

#132949

Purpose

Expresses tagless S3-M129C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

E.coli ribosomal protein S3 (rpsC E.coli)

Expression

Bacterial

Mutation

M129C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3

Plasmid

#128591

Purpose

Expresses tagless S3 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S3 E.coli ribosomal protein (rpsC E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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CMVTCRb_candidate8

Plasmid

#165006

Purpose

Expression of candidate TCR #8 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate8

Plasmid

#165005

Purpose

Expression of candidate TCR #8 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGuide-P1-O1a (EL702)

Plasmid

#140040

Purpose

CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

Depositor

Johan Elf

Article

Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

Insert

barcode P1 and sgRNA lacO1 array

Use

Synthetic Biology

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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p5343_UC57

Plasmid

#126645

Purpose

environmental plasmid p5343 cloned into pUC57

Depositor

Aindrila Mukhopadhyay

Article

Kothari et al mSystems. 2019 Dec 17;4(6). pii: 4/6/e00

Insert

Environmental plasmid p5343

Use

Synthetic Biology

Promoter

NA

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p1146-pCSDest-AcrIIC3Nme-mCherry

Plasmid

#126202

Purpose

Mammalian expression of AcrIIC3Nme fused to mCherry

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIC3Nme

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/68

Plasmid

#113921

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 68

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/68

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_MBP-nLucCP-65/66

Plasmid

#113920

Purpose

Plasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 65 and 66

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

MBP-NanoLucCP-65/66

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMEnrp1bP1gfp

Plasmid

#90129

Purpose

Gateway Mid-entry clone containing promoter sequence specific for nrpb1b driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

nrp1bP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEmafbbP1gfp

Plasmid

#90128

Purpose

Gateway Mid-entry clone containing promoter sequence specific for maffbb driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

maffbbP1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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mGeos2-VEL-Farnesyl-5

Plasmid

#57548

Purpose

Localization: Membrane, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

mGeos2-VEL

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mPapaya1-CytERM-N-17

Plasmid

#56352

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

CytERM

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Xp75 CDD

Plasmid

#61283

Purpose

Expresses Xenopus p75 CDD domain

Depositor

Mark Bothwell, Lara Hutson

Article

Hutson et al J Neurobiol. 2001 Nov 5;49(2):79-98.

Insert

p75 CDD (p75NTRa Frog)

Use

Xenopus expression

Expression

Mammalian

Mutation

lacks all but the first five amino acids of the p…

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only
mOrange2-Cx26-7

Plasmid

#57949

Purpose

Localization: Gap Junctions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx26

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
mOrange2-Cx43-7

Plasmid

#57951

Purpose

Localization: Gap Junctions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mKO2-Cx43-7

Plasmid

#57867

Purpose

Localization: Gap Junctions, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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