We narrowed to 19,203 results for: IRE

Showing: 7151 - 7200 of 19203 results

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pET19b-DAD2 cPGFP(A165)

Plasmid

#165999

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(A165)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2 cPGFP(G164)

Plasmid

#165997

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2 cPGFP(G164)

Tags

6His

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_hTLN2-FL-LBD

Plasmid

#164841

Purpose

Expression of GFP tagged FL human talin2 mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-GFP

Expression

Mammalian

Mutation

Lipid binding deficient mutations

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2

Plasmid

#165996

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2

Tags

6His

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-rShHTL7 cPGFP(L166)

Plasmid

#165995

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

rShHTL7 cPGFP(L166)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET19b-DAD2 cPGFP(G164)

Plasmid

#165993

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

DAD2 cPGFP(G164)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-2xStrep-mu68

Plasmid

#162662

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68

Plasmid

#162625

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-mu68

Plasmid

#162627

Purpose

Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL52

Plasmid

#162628

Purpose

Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJPS2951

Plasmid

#135585

Purpose

SNR6 has UU at position 105–106 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ2 has UU at position 105–106 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2954

Plasmid

#135588

Purpose

SNR6 has UAUUUCat position 102–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ6 hasUAUUUCat position 102–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2956

Plasmid

#135590

Purpose

SNR6 has GAUUUAUUUC at position 98–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ10 has GAUUUAUUUC at position 98–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJPS2957

Plasmid

#135591

Purpose

SNR6 has GAGAUUUAUUUC at position 96–107 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6Δ12 has GAGAUUUAUUUC at position 96–107 deleted

Promoter

Native

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL052

Plasmid

#137906

Purpose

OsBiP1 promoter driven Cas9-tRNA system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL053

Plasmid

#137907

Purpose

OsBiP1 promoter driven Cas9-csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1752

Plasmid

#139877

Purpose

Expression plasmid for naturally occurring 5xMCE protein (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

Nitrincola_nitratireducens_D791_00999

Expression

Bacterial

Mutation

Nitrincola_nitratireducens_D791_00999(47-644)-6xH…

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKM45

Plasmid

#154152

Purpose

Firefly luciferase with no promoter for negative control for luciferase assays

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIntegrase-AntiDsRed

Plasmid

#139677

Purpose

Cassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Integrase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Cerulean3

Plasmid

#139374

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Cerulean3 N1

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQP-iRISB

Plasmid

#138979

Purpose

Light-controlled tri-directional targeting of endogenous proteins

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL050

Plasmid

#137904

Purpose

Dual-mini35S driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL051

Plasmid

#137905

Purpose

Dual-mini35S-enhancer driven Cas9-Csy4 system

Depositor

Yong Zhang

Article

Ren et al Front Plant Sci. 2019 Sep 20;10:1173. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UL91-2xStrep pCDNA4

Plasmid

#120375

Purpose

Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL91 (UL91 Human Cytomegalovirus (HHV-5))

Tags

2xStrep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-Cdc3-Cdc10-SNAP

Plasmid

#131161

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10-SNAP

Use

Bicistronic

Tags

Cdc10: SNAP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB2629

Plasmid

#113394

Purpose

MET25p-MS2-RFP-CYC1 TT

Depositor

Suzanne Sandmeyer

Article

Clemens et al Virus Res. 2013 Feb;171(2):319-31. doi:

Insert

MS2 bacteriophage coat protein

Tags

red fluorescent protein (RFP), codon-optimized fo…

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MEI-S332-GFP genomic in pCaSpeR

Plasmid

#113174

Purpose

In P element vector for transposon insertion into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

Insert

mei-S332 (Sgo)

Use

P insertion vector

Tags

GFP

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
KG#244

Plasmid

#110927

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTC102

Plasmid

#91176

Purpose

a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:TALEN

Use

TALEN

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWK

Plasmid

#62713

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Expression

Mammalian

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pARD (pKD-G4)

Plasmid

#62683

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Promoter

Unknown

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pNB762

Plasmid

#60150

Purpose

Firefly luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB767

Plasmid

#60159

Purpose

Firefly luciferase (Promega) driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNB649

Plasmid

#60136

Purpose

Firefly luciferase (Promega) driven by MET17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

A4V

Promoter

MET17pr

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RASL12

Plasmid

#55679

Purpose

expression of mouse RASL12

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASL12 (Rasl12 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pHL 342

Plasmid

#53012

Purpose

PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp

Depositor

Han Lim

Article

Hussein et al Nucleic Acids Res. 2012 May 22.

Inserts

mCherry
araC
ompC

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -81 to +36 relative to start codon

Promoter

Plar, pLlacO-1, and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
Phamret-H2B/pcDNA3

Plasmid

#51955

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to H2B

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Tags

human H2B

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Phamret-Fibrillarin/pcDNA3

Plasmid

#51954

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein, fused to Fibrillarin for nucleolar localization

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Tags

Fibrillarin

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
CS2-GFP-nopo

Plasmid

#47964

Purpose

Expression of Fly nopo

Depositor

Laura Lee

Article

Merkle et al Development. 2009 Feb;136(3):449-59. doi

Insert

no poles (nopo Fly)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pSS63

Plasmid

#47951

Purpose

rtTA positive feedback loop with single tet operator in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

T3

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo Tcrb-V13 in situ probe

Plasmid

#45622

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Tcrb-V13 in situ probe (Tcrb Mouse)

Use

In situ

Mutation

fragment contains bp# 533-1058 of X67128

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 d44

Plasmid

#44191

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 44 amino acids

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only
Syntaxin-4 d24

Plasmid

#44189

Depositor

Thomas Weimbs

Article

Reales et al PLoS One. 2011;6(6):e21181. doi: 10.1371

Insert

Syntaxin-4

Tags

2xMyc and His

Expression

Mammalian

Mutation

deleted first 24 amino acids

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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We narrowed to 19,203 results for: IRE