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pMiNGo11-1

Plasmid

#126056

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE77

Plasmid

#80579

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p4nmTriangle

Plasmid

#250946

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 4nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

4nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p20nmTriangle

Plasmid

#250947

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 20nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

20nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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p60nmTriangle

Plasmid

#250948

Purpose

Expresses a putative triangle with dTomato-mVenus-mVenusHP-dTomato in mammalian cells. Each fluorescent protein is separated by a 60nm ER/K helix.

Depositor

Steven Vogel

Article

Nguyen et al Biophys J. 2025 Dec 2;124(23):4293-4309.

Insert

60nm Triangle

Tags

6His

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pSynITR_DD

Plasmid

#253227

Purpose

This is a DD-ITR AAV Production plasmid that contains the SynITR sequence as published in PMID: 39868534

Depositor

Matthew Hirsch

Article

Song et al Nucleic Acids Res. 2025 Jan 24;53(3):gka

Inserts

Enhanced Green Fluorescent Protein
SynITR

Use

AAV

Expression

Mammalian

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamM(pre-cut)AB

Plasmid

#248942

Purpose

Hachiman from P. fluorescens under pTet control with HamM programmed breaks in the peptide backbone at I118 and I218

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamM(pre-cut)AB

Expression

Bacterial

Mutation

HamM programmed breaks in the peptide backbone at…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(-cJun)B

Plasmid

#248940

Purpose

Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

flHamMA(-cJun)B

Expression

Bacterial

Mutation

c-Jun insertion, HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(S157A)(deltaA)B

Plasmid

#248939

Purpose

Hachiman from P. fluorescens under pTet control with the dHamA domain removed and with a protease S157A mutation

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(S157A)(deltaA)B

Expression

Bacterial

Mutation

S157A, HamA domain deletion

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTet_PflHamMA(S157A)(-cJun)B

Plasmid

#248941

Purpose

Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker and the protease mutated (S157A)

Depositor

Jennifer Doudna

Article

Tuck et al Science. 2026 Jan 8;391(6781):195-201. d

Insert

PflHamMA(S157A)(-cJun)B

Expression

Bacterial

Mutation

S157A, c-Jun insertion, HamA domain deletion

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pATDt

Plasmid

#249499

Purpose

Expresses tdTomato – Monodirectional chloroplast expression vector (psbH Rescue) in Chlamydomonas Reinhardtii photosynthetically deficient strain CC4388

Depositor

Isabel Desgagné-Penix

Insert

tdTomato (red fluorescence)

Use

Chlamydomonas reinhardtii chloroplast genome

Expression

Bacterial

Mutation

tdTomato results from the fusion of two copies of…

Promoter

The psbD/ 5′ UTR, a light-activated chloroplast p…

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pcDNA3

Plasmid

#236199

Purpose

Mammalian expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Mammalian

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRnr-ND

Plasmid

#232579

Purpose

Expression of the Rnr nuclease domain (residues 649 to 1929) as a GST-fusion.

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

Rnr nuclease domain (residues 649 to 1929)

Tags

GST

Expression

Bacterial

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDM070

Plasmid

#216816

Purpose

Aspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for C-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mCardinal

Tags

VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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