We narrowed to 159,557 results for: addgene

1. pAG415Gal_Gcn4 Aro3+GFP

   Plasmid

   #233002

   Purpose

   Galactose iduced expression of Gcn4 Aro3+GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Aro3+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103Y, N112T, L113Y, S117F, K140R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMVRha

   Plasmid

   #229553

   Purpose

   Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.

   Depositor

   Sandy Schmidt

   Article

   Vajente et al ACS Synth Biol. 2024 Oct 31. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTlpA_mCherry_YafQ/DinJ

   Plasmid

   #229148

   Purpose

   Toxin-Antitoxin (Dinj/YafQ)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, DinJ/YafQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtlpA (mCherry), DinJ/YafQ (Native)

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTlpA_mCherry_Txe/Axe

   Plasmid

   #229147

   Purpose

   Toxin-Antitoxin (Txe/Axe)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, Txe/Axe
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtlpA (mCherry), Txe/Axe (Native)

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGFP-AUA-IRES-mCherry

   Plasmid

   #222116

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AUA start codon

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-AGC-IRES-mCherry

   Plasmid

   #222119

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   AGC start codon

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZB260_pT7-STR-H3U3

   Plasmid

   #228506

   Purpose

   Bacterial Hybrid Reporter Plasmid, pT7-HIS3/URA3

   Depositor

   Timothy Whitehead

   Article

   Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

   Insert

   HIS3/URA3 operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-CUG-IRES-mCherry

   Plasmid

   #222110

   Purpose

   Start codon reporter with mutated start codon

   Depositor

   Jonathan Weissman

   Article

   She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

   Insert

   GFP-IRES-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   CUG start codon

   Promoter

   Available since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGFP-ACG-IRES-mCherry

    Plasmid

    #222113

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ACG start codon

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-UUG-IRES-mCherry

    Plasmid

    #222112

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    UUG start codon

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGFP-AUU-IRES-mCherry

    Plasmid

    #222118

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    AUU start codon

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGFP-AGG-IRES-mCherry

    Plasmid

    #222115

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    AGG start codon

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-AUC-IRES-mCherry

    Plasmid

    #222117

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    AUC start codon

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAL4-sfGFP(1-157)

    Plasmid

    #220743

    Purpose

    GAL4-sfGFP(1-157) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(1-157)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAL4-sfGFP(158-238)

    Plasmid

    #220744

    Purpose

    GAL4-sfGFP(158-238) construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-sfGFP(158-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAL4-NIR-FbLAG30

    Plasmid

    #220746

    Purpose

    GAL4-NIR-FbLAG30 construct expression in mammlian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

    Insert

    GAL4-NIR-FbLAG30
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Puro-SMCR8

    Plasmid

    #83440

    Purpose

    Expresses Cas9 and a gRNA targeting SMCR8

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL452

    Plasmid

    #219502

    Purpose

    Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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