We narrowed to 7,293 results for: 11

1. WNT11-bio-His

   Plasmid

   #53430

   Purpose

   Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   WNT11 (WNT11 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3_002

   Plasmid

   #123719

   Purpose

   Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Blasticydin Resistance
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pONSY-Venus

   Plasmid

   #111875

   Purpose

   This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ908

   Plasmid

   #44474

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, PuroR, [4-3] Gateway
   

   Use

   Gateway destination vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

   Plasmid

   #140200

   Purpose

   Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   dLbCas12a crRNA scaffold

   Promoter

   PgpdA

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-HA-REDD1 P139A (#625)

   Plasmid

   #99945

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P139A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCh-466

   Plasmid

   #92251

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C CED in mammalian cells

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690

   Promoter

   EF1A

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-REDD1 S137A (#627)

   Plasmid

   #99943

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S137A

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. RNAPII_CTD27-52_K5S

    Plasmid

    #98679

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K1838S, K1859S, K1866S, K1873S, K1887S

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSIN-Arkadia-gRNA2

    Plasmid

    #180362

    Purpose

    targeting mouse Arkadia gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Arkadia targeting gRNA (Rnf111 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPuroR-MCS-GAL4-MiniWhite

    Plasmid

    #165890

    Purpose

    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK2_016

    Plasmid

    #123708

    Purpose

    Encodes truncated pCMV as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCMV-A
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T MDM2 S166D

    Plasmid

    #16238

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser166 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pNAS1B split mCherry N-MESVD/TRAP(RK)-C

    Plasmid

    #61967

    Purpose

    Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-MESVD
    TRAP(RK)-C-mCherry
    

    Expression

    Bacterial

    Promoter

    PBAD and PLtetO

    Available Since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH2037

    Plasmid

    #179220

    Purpose

    Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    UNC-7 (unc-7 Nematode)
    

    Tags

    wCherry

    Expression

    Worm

    Promoter

    Psra-11

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. MEIS1B-pSP65

    Plasmid

    #21019

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1B (Meis1 Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

18. RNAPII_CTD38-52

    Plasmid

    #98685

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 38-52, residues 1853-1970

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HOXD12 (murine) pRSETa

    Plasmid

    #8554

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Tags

    HIS and XPress

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. MTK4b_004

    Plasmid

    #123843

    Purpose

    Encodes spacer sequence as a Type 4b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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