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TOPO HygroR

Plasmid

#68445

Purpose

TOPO construct expressing HygroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hygromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TOPO ZeoR

Plasmid

#68446

Purpose

TOPO construct expressing ZeoR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Zeocin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO PuroR

Plasmid

#68447

Purpose

TOPO construct expressing PuroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

Plasmid

#214138

Purpose

Insect cell expression of human 20S CP alpha-subunits

Depositor

Brenda Schulman

Publication

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)
PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Promoter

polyhedrin

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1_SPTLC1_C133W

Plasmid

#181921

Purpose

3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W

Depositor

Christian Metallo

Publication

Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

Insert

Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)

Use

Lentiviral; Mammalian expression, , doxycycline i…

Expression

Mammalian

Mutation

cysteine at position 133 of the coding region of …

Promoter

tight TRE promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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PM-RA

Plasmid

#209878

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-Intb2

Plasmid

#205194

Purpose

This vector encodes of a synCAM (intb2) and GFP

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb2 and GFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Publication

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Gate2 ccdB

Plasmid

#117116

Purpose

Destination vector

Depositor

Stephan Gruber

Publication

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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SIV_Rev

Plasmid

#236244

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Rev

Use

Lentiviral

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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PMT-mKate2

Plasmid

#203785

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mKate2

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mWasabi-G

Plasmid

#164161

Purpose

Expresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Publication

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Expression

Mammalian

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C77A

Plasmid

#133889

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Publication

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Expression

Mammalian

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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mSc-sspB

Plasmid

#114421

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Publication

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pOGG024

Plasmid

#113991

Purpose

Level 1 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCoofy5

Plasmid

#43989

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-Sumo1

Expression

Bacterial

Promoter

T7

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Perox-GA

Plasmid

#209868

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1B

Plasmid

#202722

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B (PIP5K1B Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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