We narrowed to 9,185 results for: Pol;

1. CMV-CDX2-MUT

   Plasmid

   #16554

   Depositor

   Bert Vogelstein

   Article

   da Costa et al Oncogene. 1999 Sep 2. 18(35):5010-4.

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Mutation

   RKO mutation

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pCellFree_G03 IL2

   Plasmid

   #67053

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   IL2 (IL2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRK5myc Cdc42 L61

   Plasmid

   #15906

   Depositor

   Alan Hall

   Article

   Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

   Insert

   Cdc42 (placental isoform) (CDC42 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Q61L

   Available Since

   Nov. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pRK5myc Cdc42 N17

   Plasmid

   #15907

   Depositor

   Alan Hall

   Article

   Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

   Insert

   Cdc42 (placental isoform) (CDC42 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T17N

   Available Since

   Nov. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pIS1 Cyclin D2 long UTR

   Plasmid

   #21645

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 long UTR (CCND2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3XFlag DVL2 (∂PY)

   Plasmid

   #24808

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   DVL2 (DVL2 Human)
   

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of the PY motif: PPPY

   Available Since

   June 22, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pET21a - APX

   Plasmid

   #89618

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Ascorbate dependent Tryparedoxin peroxidase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB8792

   Plasmid

   #126910

   Purpose

   Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   ADE2 gRNA
   

   Use

   Synthetic Biology

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSV(X) C/EBPbeta

   Plasmid

   #13393

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPbeta (Cebpb Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

10. CBla_2.0_EmrE_TMD4_3P

    Plasmid

    #207848

    Purpose

    BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

    Depositor

    Dieter Langosch

    Article

    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

    Insert

    C-BLa 2.0 fusion protein
    

    Tags

    Flag (DYKDHDG)

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSicoR-mCh-GFPi

    Plasmid

    #21905

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    GFPi
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE flag M33

    Plasmid

    #1951

    Depositor

    Robert Kingston

    Article

    Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

    Insert

    M33 (Cbx2 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pGEMHE-mClover3

    Plasmid

    #171497

    Purpose

    T7 promotor drives in vitro transcription of mClover3 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    mClover3
    

    Use

    Pgehme

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSV(X) C/EBPalpha

    Plasmid

    #13392

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPalpha (Cebpa Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

15. TRF2-HA

    Plasmid

    #200209

    Purpose

    Expression of full length mTRF2

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TRF2-HA (Terf2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH-HT_CtCHAD

    Plasmid

    #127785

    Purpose

    Expresses CtCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3XFlag DVL2 (E499-G)

    Plasmid

    #24803

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    DVL2 (DVL2 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    E499G

    Available Since

    June 22, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pPRIME-TET-GIN-recipient

    Plasmid

    #11656

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP, IRES, and Neo

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJin-290

    Plasmid

    #125718

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    

    Expression

    Mammalian

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFastBac K-M33

    Plasmid

    #1986

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    M33 (Cbx2 Mouse)
    

    Expression

    Insect

    Mutation

    Kozak sequence added before ATG

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pSCALPS_miR-146aSponge_mCherry_GFP

    Plasmid

    #149718

    Purpose

    Lentiviral vector expressing a miR-146a decoy sponge

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    14X miR-146a binding sites
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G to T mutation in position 75 of the insert

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. psicheck-2-Tas2r3

    Plasmid

    #48176

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of TAS2R3 (TAS2R3 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pX330(puro) Rosa26-H3F3B

    Plasmid

    #73131

    Purpose

    Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

    Depositor

    Agnel Sfeir

    Article

    Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

    Insert

    spCas9
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX4T-1-human RNF146 RING-WWE (30-183)

    Plasmid

    #132611

    Purpose

    expresses human RNF146 RING-WWE (30-183) in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

    Insert

    RNF146 RING-WWE (30–183)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pMX156

    Plasmid

    #29482

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    Kif2A-beta (Kif2a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBAD-DiB2

    Plasmid

    #168801

    Purpose

    Fluorogen activating protein (FAP) DiB2

    Depositor

    Alexander Mishin

    Article

    Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

    Insert

    Fluorogen activating protein DiB2
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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