We narrowed to 18,759 results for: nls

Showing: 5801 - 5840 of 18759 results

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TDP-43_CTD_del321-343

Plasmid

#98676

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343

Depositor

Nicolas Fawzi

Publication

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

del321-343

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-HA-NEK7 K64M

Plasmid

#75143

Purpose

Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Lys 64 mutated to Met; kinase inactive and F236L …

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAGGS-HHIP

Plasmid

#261261

Purpose

Expresses mouse HHIP under CAG promoter.

Depositor

Cliff Tabin

Publication

Yan et al Nat Commun. 2025 Nov 27;16(1):10643. doi

Insert

Hedgehog-Interacting Protein (Hhip Mouse)

Expression

Mammalian

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDIV490

Plasmid

#177698

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIV488

Plasmid

#177696

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hnRNPA2_LC_nocharge

Plasmid

#139118

Purpose

express hnRNPA2 LC with no charged residues

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all charged residues are changed to S/Q except fo…

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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kank3_pJet1.2

Plasmid

#121981

Purpose

for generating kank3 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank3 (kank3 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank4_pJet1.2

Plasmid

#121982

Purpose

for generating kank4 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Publication

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank4 (kank4 Zebrafish)

Use

Cloning vector

Promoter

T7

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mTdt

Plasmid

#87665

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Publication

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Promoter

CAG

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-mTdt

Plasmid

#87667

Purpose

retroviral vector encoding cre dependent expression of membrane Tdtomato

Depositor

Oscar Marin

Publication

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-Tdtomato

Use

Retroviral

Tags

palmitylation

Promoter

Synapsin

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Publication

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Publication

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSθ

Plasmid

#68289

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 2xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSβ

Plasmid

#68283

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 1xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S218TAG

Plasmid

#68301

Purpose

N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)

Tags

His and MBP

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-TANGO1S^CC1

Plasmid

#256129

Purpose

Expresses GFP-tagged human TANGO1S with the CC1 domain deleted

Depositor

Nicola Stevenson

Publication

Lawrence et al J Cell Sci. 2026 Oct 2:jcs.265001. doi:

Insert

TANGO1S^CC2(with TEER) (MIA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

CC1 domain deleted aa Q103-T284

Promoter

CMV

Available since

Oct. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-TANGO1S^TEER

Plasmid

#256131

Purpose

Expresses GFP-tagged human TANGO1S with the TEER domain deleted

Depositor

Nicola Stevenson

Publication

Lawrence et al J Cell Sci. 2026 Oct 2:jcs.265001. doi:

Insert

TANGO1S^TEER (MIA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

TEER domain deletion - amino acid 142-183 deleted

Promoter

CMV

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-TANGO1S

Plasmid

#256126

Purpose

Expresses GFP-tagged human TANGO1S

Depositor

Nicola Stevenson

Publication

Lawrence et al J Cell Sci. 2026 Oct 2:jcs.265001. doi:

Insert

TANGO1S (MIA3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-TANGO1S^PRD

Plasmid

#256127

Purpose

Expresses GFP-tagged human TANGO1S with the PRD domain deleted

Depositor

Nicola Stevenson

Publication

Lawrence et al J Cell Sci. 2026 Oct 2:jcs.265001. doi:

Insert

TANGO1S^PRD (MIA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

PRD domain deleted - amino acids 528-779

Promoter

CMV

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_D290V

Plasmid

#98663

Purpose

Expresses MBP-tagged full length hnRNPA2 with D290V

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

D290V

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-hnRNPA2_FL_P298L

Plasmid

#104468

Purpose

express MBP-tagged full length hnRNPA2 P298L

Depositor

Nicolas Fawzi

Publication

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His-MBP

Expression

Bacterial

Mutation

P298L

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLVXNeo_BGN-SBP-mSc

Plasmid

#234884

Purpose

Expresses mScarlet-i and SBP tagged human biglycan for protein visualisation and use in the RUSH trafficking system

Depositor

Nicola Stevenson

Publication

Thompson et al J Cell Biol. 2025 Oct 6;224(10):e2024111

Insert

Biglycan (BGN Human)

Use

Lentiviral

Tags

SBP and mScarleti

Expression

Mammalian

Mutation

See Depositor Comments below

Promoter

CMV

Available since

March 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1276

Plasmid

#111115

Purpose

Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51C gRNA (RAD51C Human)

Use

Lentiviral

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1161

Plasmid

#111097

Purpose

Expresses FANCA_-_89883045.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCA gRNA (FANCA Human)

Use

Lentiviral

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_A321G

Plasmid

#98670

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

Depositor

Nicolas Fawzi

Publication

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A321G

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-TetOn-puro-ICP8

Plasmid

#250616

Purpose

Mammalian lentiviral vector for the inducible (TetOn) expression of codon-optimized HSV-1 ICP8

Depositor

Liam Holt

Publication

Herzog et al Mol Cell. 2026 Mar 5;86(5):817-833.e7. d

Insert

ICP8

Use

Lentiviral

Expression

Mammalian

Mutation

Codon-optimized for Homo sapiens

Promoter

TRE3GS promoter

Available since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_P298L

Plasmid

#139111

Purpose

Express FL hnRNPA2 P298L with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Publication

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

P298L

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe mouse Bmf

Plasmid

#17241

Depositor

Joan Brugge

Publication

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Publication

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Expression

Bacterial

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP43-NTD

Plasmid

#241024

Purpose

Bacterial expression of TDP-43 NTD

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP43-NTD

Tags

6x His

Expression

Bacterial

Mutation

changed N81 to stop codon

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway Cloning

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway Cloning

Mutation

E179K

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ4M/TDP-43 S48E

Plasmid

#104481

Purpose

bacterial expression of TDP-43 S48E

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Mutation

S48E

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 18,759 results for: nls