We narrowed to 9,660 results for: Gnas
-
Plasmid#74063PurposepZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanRDepositorInsertdcas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPropionate inducible promoter (prp)Available sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-WNT3A
Plasmid#108345Purposesynchronize trafficking of WNT3A from the ER (RUSH System)DepositorInsertWNT3A, Wnt family member 3A (WNT3A Human)
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
myr-palm_2xmEYFP
Plasmid#127811PurposeHomo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signalDepositorInsertmyristoylated-palmitoylated-mEYFP-mEYFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-acuR
Plasmid#62569PurposeAcrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.DepositorInsertCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJOG824
Plasmid#105401PurposeSynthetic biologyDepositorInsertSV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceMarch 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
UseTagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastMutationPromoterTPI1Available sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-R-GFP
Plasmid#11953DepositorInsertUb-R-GFP
UseTagsExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Arginine at…PromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
myr-palm_mCherry-mEGFP
Plasmid#127815PurposeHetero-dimer mCherry-mEGFP expression vector with plasma membrane localization signalDepositorInsertmyristoylated-palmitoylated-mCherry-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_TEV
Plasmid#58868PurposeMESA protease chain with mCherry ectodomain and no linker domainDepositorInsertMESA protease chain with mCherry ectodomain and TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)
Plasmid#70745Purposefor building 3-color reporter plasmidDepositorInsertmCherry-ssrA
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)
Plasmid#70742Purpose1 color weak reporter used in studyDepositorInsertsfGFP-ssrA
UseTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCaf-mCherry
Plasmid#192860PurposepMUT2 based vector for sensing caffeine in E. coli Nissle 1917DepositorInsertNanobody-CadC fusion protein and cognate promoter inducible by caffeine
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Kma-Inulinase SP
Plasmid#153492PurposeKluyveromyces marxianus Inulinase Signal Peptide Donor PlasmidDepositorInsertInulinase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXA9-MSCV-full-GFP
Plasmid#20977DepositorInsertHOXA9 (Hoxa9 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationFull length cDNA with ~1.1kb 3'UTR with 2 pu…PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_250/pcDNA3
Plasmid#85206Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_250
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME nls-eos
Plasmid#73791PurposeMiddle entry clone with photoconvertible eos conjugated to a nuclear localization signalDepositorInsertnls-eos
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate1
Plasmid#31873DepositorInsertNLS-LSS-mKate1
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-FLAG-ER-5
Plasmid#170622PurposeExpresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-PesaR-Clux
Plasmid#47658PurposePesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)DepositorInsertPesaR-C
UseSynthetic BiologyTagsExpressionBacterialMutationAdditional esa box between -35 site and the origi…PromoterPesaR-CAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLS-LSSmKate1-N1
Plasmid#31870DepositorInsertNLS
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterCMVAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJOG140
Plasmid#105399PurposeSynthetic biologyDepositorInsertPKI nuclear export signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG141
Plasmid#105400PurposeSynthetic biologyDepositorInsertSV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNa-delta S
Plasmid#107983Purposebacterial expression of full-length OPNDepositorInsertOPNa 1080bp-17aa signal (SPP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLS-LSSmKate2-N1
Plasmid#31871DepositorInsertNLS
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…PromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
EPHB3
Plasmid#28165PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEPHB3 (EPHB3 Human)
UseTagsHis tag with TEV cleavage and Honeybee melittin S…ExpressionBacterialMutationLigand binding domain (residues 39-211).PromoterAvailable sinceMarch 18, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
Myc-gamma3 (in pCDNA3.1)
Plasmid#118041PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCDNA3.1)
Plasmid#118038PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCAGGS)
Plasmid#118042PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNb-delta S
Plasmid#107984Purposebacterial expression of OPN splice variant bDepositorInsertOPNb 1038bp-17aa signal (SPP1 Human)
UseTagsGSTExpressionBacterialMutationWT splice variantPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-OPNc-delta S
Plasmid#107985Purposebacterial expression of OPN splice variant cDepositorInsertOPNc 999bp-16aa signal (SPP1 Human)
UseTagsGSTExpressionBacterialMutationWT splice variantPromoterAvailable sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
GPKAnls
Plasmid#61094PurposeGFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICH44300
Plasmid#50340DepositorInsert3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
GPKAnes
Plasmid#61092PurposeGFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signalDepositorInsertpkac2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_002
Plasmid#123841PurposeEncodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK systemDepositorInsertSV40 pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPTK007-3a-αMFΔ
Plasmid#84966PurposeType 3a, α-mating factor pre-sequence shortened - secretion signalDepositorInsertα-mating factor, pre-sequence shortened secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK008-3a-αMFΔ no Kex
Plasmid#84967PurposeType 3a, α-mating factor no Kex recognition site - secretion signalDepositorInsertα-mating factor no Kex recognition site secretion tag
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GHRHR
Plasmid#39173PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertGHRHR (GHRHR Human)
UseBaculovirus expressionTagsSignal-His6-TEVExpressionMutationN20->Q mutation of putative glycosylsation sit…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_TEV
Plasmid#58870PurposeMESA protease chain with CD4 ectodomain and no linker domainDepositorInsertMESA protease chain with CD4 ectodomain and TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAWp7-10
Plasmid#72742PurposepFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]DepositorInsertUBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGL1010
Plasmid#119081PurposeIntroducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).DepositorTypeEmpty backboneUseFungal expression (in magnaporthe oryzae)TagsExpressionMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_003
Plasmid#123842PurposeEncodes rabbit beta-globin polyadenylation signal as a Type 4b part to be used in the MTK systemDepositorInsertrglb pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NLS KOZ c2
Plasmid#54443DepositorInsertNuclear localization signal (NLS) of SV40 large T antigen
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma2s-EGFP
Plasmid#118040PurposeGABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminusDepositorInsertGABRG2 (Gabrg2 Rat)
UseTagsEGFP and cMyc epitope EQKLISEEDL inserted between…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only