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We narrowed to 12,214 results for: LEA

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Showing: 2901 - 2920 of 12214 results

  1. p2B-56

    Plasmid
    #177925
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Depositor
    Vadim Gladyshev
    Publication
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    MYO2
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177925-plasmid-map-sequence-id-349995

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  7. pSH-Csy4-T2A-SaRFN

    Plasmid
    #85755
    Purpose
    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-SadCas9
    Use
    CRISPR
    Tags
    3xHA tag, Csy4, and FokI fusion via 36 amino acid…
    Expression
    Mammalian
    Mutation
    D10A, N580A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85755-plasmid-map-sequence-id-170274

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  8. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Publication
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  9. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165540-plasmid-map-sequence-id-339262

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  10. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Publication
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    177334-plasmid-map-sequence-id-348602

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  11. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Publication
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    175276-plasmid-map-sequence-id-349086

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  13. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid
    #118407
    Purpose
    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR-NES
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118407-plasmid-map-sequence-id-230143

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  20. pcDNA3.1(+)-Mito-CaNARi

    Plasmid
    #64851
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaNARi
    Tags
    Cerulean, N-terminal 30 amino acids from DAKAP1, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64851-plasmid-map-sequence-id-109676

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Showing: 2901 - 2920 of 12214 results