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LoxP1-Mb2YFP-2A (SO244)

Plasmid

#99613

Purpose

To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_Q62C-A140C

Plasmid

#162581

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Glutamine 62 changed to Cysteine and Alanine 140 …

Promoter

polyhedring

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK JunD-HA neo

Plasmid

#58515

Purpose

Lentiviral expression vector for an inducible HA-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pLenti-HF1RA-PGK-Puro

Plasmid

#110860

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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CK2alpha V66A/I174A-HA (pJD23)

Plasmid

#37362

Depositor

David Litchfield

Article

Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

Insert

CK2α V66A/I174A (CSNK2A1 Human)

Tags

HA X3

Expression

Mammalian

Mutation

V66A, I174A

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pLX302 PCSK5-V5 puro

Plasmid

#98389

Purpose

Lentiviral vector for constitutive expression of human PCSK5 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-H169Q-GFP

Plasmid

#78917

Purpose

Mammalian expression of BAP1 with H169Q and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

H169Q

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Control-cx43.4-48H-pPRISM

Plasmid

#117801

Purpose

Control to test technique on a target that is known to work

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)-mNeonGreen

Plasmid

#205021

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT-mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Shank [N23B/49R]

Plasmid

#128637

Purpose

Mammalian Expression Plasmid of anti-Pan-Shank. Derived from hybridoma N23B/49.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Shank Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_BirA_CDKN1A

Plasmid

#136538

Purpose

Lentiviral expression vector for an inducible BirA-CDKN1A

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA-CDKN1A (CDKN1A )

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-C91G-GFP

Plasmid

#78916

Purpose

Mammalian expression of BAP1 with C91G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91G

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
sgRNA BRCA1 C64Y

Plasmid

#139326

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 C64Y using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-4* [N98/7R]

Plasmid

#140081

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-4* (Mouse). Derived from hybridoma N98/7.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-4* (Mouse) recombinant mouse monoclonal antibody (Nlgn4l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-hMSN

Plasmid

#211823

Purpose

Express human MSN in mammalian cells

Depositor

Haian Fu

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-Puro

Plasmid

#110853

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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HA-CK2alpha’ V67A/I175A (pJD24)

Plasmid

#37363

Depositor

David Litchfield

Article

Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

Insert

CK2α' V67A/I175A (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Mutation

V67A, I175A

Promoter

CMV

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-mtagBFP-2A RIGI K270A

Plasmid

#167290

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

K270A

Promoter

SFFV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.1.mKO2

Plasmid

#85209

Purpose

TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hGRM1-RFP

Plasmid

#22920

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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