We narrowed to 4,951 results for: mcs

1. pAAV-CAG-DIO-ChR2(H134R)-eYFP

   Plasmid

   #127090

   Purpose

   Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

   Depositor

   Viviana Gradinaru

   Article

   Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

   Has Service

   AAV PHP.eB

   Insert

   Channelrhodopsin-2
   

   Use

   AAV

   Tags

   eYFP (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Humanized ChR2 gene with histidine 134 changed to…

   Promoter

   CAG

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFPC1-hVAP-A KD/MD

   Plasmid

   #104449

   Purpose

   Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein A (VAPA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed K94 to D and M96 to D

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

   Plasmid

   #223273

   Purpose

   Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170995

   Purpose

   HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HH02-LP Clone 2 heavy chain

   Plasmid

   #192175

   Purpose

   Clone 2 heavy chain (HH02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 heavy chain (HH02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HK02-LP Clone 2 light chain

   Plasmid

   #192179

   Purpose

   Clone 2 light chain (HK02)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 2 light chain (HK02)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

   Plasmid

   #212522

   Purpose

   Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMMB67EH-dddAI

   Plasmid

   #186559

   Purpose

   Broad host range regulated expression of the DddA immunity determinant (DddAI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Spike-deltaC18 LB.1

   Plasmid

   #223277

   Purpose

   Encodes SARS-CoV-2 spike LB.1 for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike LB.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGC1a_Proximal Promoter-FLuc2P

    Plasmid

    #154259

    Purpose

    Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Proximal Promoter (PPARGC1A Human)
    

    Expression

    Mammalian

    Promoter

    Human PGC-1alpha Proximal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR-blunt-TOPO-FNDC5

    Plasmid

    #35970

    Depositor

    Bruce Spiegelman

    Article

    Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

    Insert

    fibronectin type III domain containing 5 (Fndc5 Mouse)
    

    Use

    Cloning vector

    Available Since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-PPO-mScarlet

    Plasmid

    #139503

    Purpose

    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    parapinopsin
    

    Tags

    mScarlet following Gly-Gly-Ser linker

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPGC1a_Distal Promoter-FLuc2P

    Plasmid

    #154260

    Purpose

    Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    PGC-1a Distal Promoter (PPARGC1A Human)
    

    Expression

    Mammalian

    Promoter

    Human PGC-1alpha Distal Promoter

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC1-hVAP-B KD/MD

    Plasmid

    #104450

    Purpose

    Expression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein B (VAPB Human)
    

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    changed K87 to D and M89 to D

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT311 AAV-Rapamycin Inducible-NZF-Citrine

    Plasmid

    #202060

    Purpose

    Inducible AAV activator

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    AAV-Rapamycin Inducible-NZF-Citrine
    

    Use

    AAV; Aav with reporter

    Expression

    Mammalian

    Mutation

    ITR deletion

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-KIF1B beta-FL

    Plasmid

    #79108

    Purpose

    Mammalian expression of GFP-KIF1B beta-FL

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta full length (KIF1B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

19. lentiCRISPR v2-sgBAP1-1

    Plasmid

    #125837

    Purpose

    KO BAP1 gene

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

    Plasmid

    #223272

    Purpose

    Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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