We narrowed to 4,848 results for: pAAV

1. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

   Plasmid

   #74306

   Purpose

   Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Inserts

   TRPV4 (Trpv4 Rat)
   ferritin (FTL and HTL1) (FTL, FTH1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG and p2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (after cre recombination)

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

   Plasmid

   #83489

   Purpose

   cre-dependent PAK1-AID under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   PAK1-AID-ires-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

   Plasmid

   #136916

   Purpose

   Expresses ChR2(H134R)-EYFP in Cre-negative cells

   Depositor

   Ofer Yizhar

   Article

   Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H134R in ChR2

   Promoter

   Human Synapsin1

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Dlx5/6-Chronos-eGFP-p2a-nls-mScarlet

   Plasmid

   #121543

   Purpose

   Chronos with red nucleus-targeted fluorescence for inhibitory neurons

   Depositor

   Matthias Prigge

   Insert

   blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   dlx5/6

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

   Plasmid

   #214265

   Purpose

   Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

   Depositor

   D. James Surmeier

   Insert

   Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Synapsin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

   Plasmid

   #83490

   Purpose

   cre-dependent CA-AMPK under EF1a promoter

   Depositor

   Dong Kong

   Article

   Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

   Insert

   CA-AMPK-2A-mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   H150R

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

   Plasmid

   #52494

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Foff 2.0-bREACHes-EYFP

   Plasmid

   #137146

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Con/Foff 2.0-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #171117

   Purpose

   Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   AAV

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   gfaABC1D

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

    Plasmid

    #125225

    Purpose

    Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47455

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    AAV

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

    Plasmid

    #204852

    Purpose

    AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd6-nSaCas9KKH(D10A)-UGI
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    D10A, E782K, N968K, R1015H in SaCas9

    Promoter

    EFS, miniU6

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP

    Plasmid

    #137150

    Purpose

    Intersectional viral expression of Arch3.3-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-Arch3.3-p2a-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

    Plasmid

    #184633

    Purpose

    Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Aldh1a1 (Aldh1a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA

    Plasmid

    #47456

    Purpose

    LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE(Grm2)-GS-CIB1(NLS*,∆318-334)
    

    Use

    AAV; Tale

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

    Plasmid

    #111387

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid

    #176276

    Purpose

    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

    Plasmid

    #137143

    Purpose

    Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-ChR2-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    H134R

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109311

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Insert

    Ldlr gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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