We narrowed to 6,488 results for: cki

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PH-mCherry-FRBx2

Plasmid

#193570

Purpose

Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and mCherry

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2S31A

Plasmid

#174332

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CPL2g/pDONR221

Plasmid

#172093

Purpose

Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Use

Gateway entry vector

Promoter

CPL2

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-NKD2S31A

Plasmid

#174331

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154169

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154170

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154171

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154172

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3

Plasmid

#154182

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLX208-SAE1

Plasmid

#171012

Purpose

Ectopic expression of SAE1

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

untagged SAE1

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLX208-nfBFP

Plasmid

#171013

Purpose

Ectopic expression of nonfluorescent BFP

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

untagged nfBFP

Use

Lentiviral

Expression

Mammalian

Mutation

BFP[H66A]

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAshort

Plasmid

#153547

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 603-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAmut

Plasmid

#153548

Purpose

Used as a donor vector for the targeted knock-in of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

a 3-bp mutation in exon 6

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Expression

Bacterial

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJS249

Plasmid

#134146

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

pHis-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ELK1_iso1

Plasmid

#135737

Purpose

Donor vector for 3' FLAG tag of human ELK1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ELK1_iso1 Homology arms (ELK1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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