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Showing: 5841 - 5880 of 10096 results

  8. pINQ-ON10

    Plasmid
    #198689
    Purpose
    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)
    Depositor
    Gualberto González Sapienza
    Article
    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d
    Insert
    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    Use
    Tags
    6xHis tag, HA tag, OmpA signal peptide, and Ribos…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    198689-plasmid-map-sequence-id-402802

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  14. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212830
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212830-plasmid-map-sequence-id-434915

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  15. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid
    #212831
    Purpose
    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.
    Depositor
    Wolfgang Enard
    Article
    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:
    Insert
    ZIM3-KRAB-dCas9-P2A-mCherry
    Use
    Tags
    HA-tag and ZIM3-KRAB
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG, TetOn
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212831-plasmid-map-sequence-id-434913

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  27. pLVX-mCherry-KATNA1, V55D

    Plasmid
    #180651
    Purpose
    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    KATNA1 (KATNA1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    siRNA resistant to: GGACAGCACUCCCUUGAAA
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180651-plasmid-map-sequence-id-358612

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  29. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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  30. Sniper2P

    Plasmid
    #193857
    Purpose
    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193857-plasmid-map-sequence-id-382456

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Showing: 5841 - 5880 of 10096 results