We narrowed to 10,815 results for: HA

1. mApple-M11-92

   Plasmid

   #134466

   Purpose

   M11-92 expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin Amino Acids 1-92 (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M1 Spastin Amino Acids 1-92

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS423-mitoT

   Plasmid

   #174525

   Purpose

   Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   Yeast mitochondrial intermembrane tether
   

   Use

   Synthetic Biology

   Tags

   eGFP

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)mGAT1YFP8

   Plasmid

   #41669

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; 8 C-terminal-m…

   Promoter

   CMV

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RBM38

   Plasmid

   #155899

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RBM38 (RBM38 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RSRC1

   Plasmid

   #155983

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RSRC1 (RSRC1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMTB2-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80066

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19-E3CY56

   Plasmid

   #103075

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
   

   Use

   CRISPR; Cloning vector

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMTB2-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80067

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTET2-lucCP

   Plasmid

   #198353

   Purpose

   Part of Renilla reporter system, encodes Firefly luciferase

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTET2

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a(CTLA4)

    Plasmid

    #177905

    Purpose

    Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CTLA4 (aa 183-223 only) (CTLA4 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. -53 bp human IFN-gamma luc

    Plasmid

    #17597

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    IFN gamma promoter (IFNG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -53 to +70 of promoter

    Promoter

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

12. PPAPDC2

    Plasmid

    #155836

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPAPDC2 (PLPP6 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mTFP1-Farnesyl-5

    Plasmid

    #55481

    Purpose

    Localization: Plasma Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. nAChR beta2 CFP

    Plasmid

    #15106

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    beta2-CFP-M (Chrnb2 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET28a(DAP10)

    Plasmid

    #177904

    Purpose

    Bacterial expression of his10-tagged human DAP10 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    DAP10 (aa 70-92 only) (HCST Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-E6MBW6

    Plasmid

    #103102

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-mCherry-p2A-LIC1 Y101A, Y103A, W120A

    Plasmid

    #74606

    Purpose

    expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 Y101A, Y103A, W120A (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Y101A, Y103A, W120A

    Promoter

    SFFV

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF713-pEGFP-N1-Hs-FL-METTL20 wt

    Plasmid

    #85114

    Purpose

    transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-7 asyn S129G

    Plasmid

    #36049

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129G (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129G

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLCHKO_GFP_Luciferase

    Plasmid

    #155079

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80060

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mPapaya1-Farnesyl-5

    Plasmid

    #56650

    Purpose

    Localization: Membrane, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPapaya1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-CHRFAM7ADelta2bp

    Plasmid

    #62515

    Purpose

    mammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)

    Depositor

    Henry Lester, Sherry Leonard

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7ADelta2bp (CHRFAM7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partially duplication of CHRNA7 with 2-bp deletio…

    Promoter

    CMV

    Available Since

    March 20, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. nAChR alpha4 CFP

    Plasmid

    #15244

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    alpha4-CFP-M (Chrna4 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

28. pF714-pET28a-Hs-FL-METTL20-NHis wt

    Plasmid

    #85115

    Purpose

    expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

29. PB-hSyn-mSyp-RT1-24xGCN4

    Plasmid

    #106300

    Purpose

    The plasmid encodes mouse Synaptophysin sparsely labeled with SunTag24x_v4.

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    mSyp-RT1-24xGCN4 (Syp Mouse)
    

    Use

    Tags

    24xGCN4

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pF711-pET28a-Hs-delta25-MCAD-NHis wt

    Plasmid

    #85112

    Purpose

    expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta25) MCAD (ACADM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PABPN1L

    Plasmid

    #155807

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPN1L (PABPN1L Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pF708-pET28a-Hs-delta38-METTL20-NHis wt

    Plasmid

    #85109

    Purpose

    expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta38) METTL20 (METTL20 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid

    #205444

    Purpose

    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-HLA-E SCT-hCD55
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. LZRS ERTm RasN17

    Plasmid

    #21198

    Depositor

    Paul Khavari

    Article

    Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

    Insert

    HRas (Esr1, HRAS Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    S17N ERTm bx3

    Promoter

    Available Since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only

35. SLC9A3R1_PDZ_2

    Plasmid

    #103933

    Purpose

    Protein expression and purification of SLC9A3R1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pENTR3c GW CHD5

    Plasmid

    #68869

    Purpose

    GW entry vector for CHD5

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

37. PTPN13_PDZ_1

    Plasmid

    #103919

    Purpose

    Protein expression and purification of PTN13 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PTPN13_PDZ_3

    Plasmid

    #103921

    Purpose

    Protein expression and purification of PTN13 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMT-flk1-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80056

    Purpose

    Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    flk1

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge