We narrowed to 3,344 results for: actin
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Plasmid#229972PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and HygroDepositorInsertActBT2ATetP2AP - H
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2AmcherryP2AP -TagBFPH
Plasmid#229971PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-HygroDepositorInsertActBT2AmcherryP2AP -TagBFPH
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
1993_pCCLsin.PPT.hPGK.KaedeCETN1.PRE
Plasmid#231804PurposeLabel centrosomes with lentivirus by encoding a fusion protein consisting of the photo-convertible protein Kaede fused to Centrin 1 (CETN1), an integral component of centrosomes.DepositorAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-L61G-pEGFP-C1
Plasmid#177426PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
TagsVenusExpressionMammalianMutationLeucine 61 replaced with glycinePromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-GG153AA-pEGFP-C1
Plasmid#177427PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
TagsVenusExpressionMammalianMutationGlycines 153 and 154 mutated to alaninePromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-G154STOP-pEGFP-C1
Plasmid#177428PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
TagsVenusExpressionMammalianMutationGlycine 154 replaced with STOP codonPromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+AtAct2t
Plasmid#170882PurposePhytobrick - AtACT2tDepositorInsertAtACT2t
ExpressionPlantMutation426C>T to remove BbsI restriction siteAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-myc-Rem2 R236A/L263A
Plasmid#51589Purposeexpresses RNAiR myc-tagged Rem2 non-Ca channel interactingDepositorInsertRem2 (Rem2 Mouse)
TagsmycExpressionMammalianMutationR236A and L263A; silent mutations at shRNA 1 and …PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S334A
Plasmid#51588Purposeexpresses RNAiR myc-tagged Rem2 non-14-3-3 interactingDepositorInsertRem2 (Rem2 Mouse)
TagsmycExpressionMammalianMutationS69A and S334A; silent mutations at shRNA 1 and 2…PromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
GMIP
Plasmid#32046PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGMIP (GMIP Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 80-357 onlyAvailable SinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-Tollip
Plasmid#178050PurposeGFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.DepositorAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag mPTIP
Plasmid#15557DepositorAvailable SinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-WIPI-1
Plasmid#38272DepositorInsertWD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
UseRetroviralTagsEGFPExpressionMammalianAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FIP3
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialPromoterT7Available SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tom20-CIB-GFP
Plasmid#117242PurposeLight activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCIBN
TagsGFPExpressionMammalianPromoterCMVAvailable SinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6
Plasmid#176652PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct-PE2-HygroR
Plasmid#149552PurposeExpression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.DepositorInsertPE2
ExpressionInsectPromoterAct5cAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-p18-CIBN
Plasmid#188990PurposeA lentiviral plasmid encoding lysosome-tagged CIBNDepositorAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only