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FUG-Pten

Plasmid

#140732

Purpose

Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Publication

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-Pten (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

hUbiC

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pmNFH

Plasmid

#85178

Purpose

Mouse neurofilament protein H cDNA mammalian expression construct

Depositor

Anthony Brown

Publication

Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

Insert

mouse NFH (Nefh Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCEV-G3-Km

Plasmid

#46817

Purpose

TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and GAL10

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Publication

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CXCR4

Plasmid

#98947

Purpose

Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4

Depositor

Erik Procko

Publication

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG (dykdddd) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-R-GECO

Plasmid

#109374

Purpose

Expresses calcium indicator R-GECO in muscle cells

Depositor

Nenad Bursac

Publication

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

R-GECO

Use

Lentiviral

Expression

Mammalian

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-1

Plasmid

#90325

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

Depositor

Andrew Goodman

Publication

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Publication

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AMPH1-BAR-mCherry

Plasmid

#85130

Purpose

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry (AMPH Human)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

Plasmid

#68337

Purpose

Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

Depositor

Feng Zhang

Insert

St3Cas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CBh

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti4/TO-hEPO

Plasmid

#50437

Purpose

express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

Depositor

Juan Melero-Martin

Publication

Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

Insert

EPO (EPO Human)

Use

Lentiviral; Tetracycline-regulatable (t-rex)

Expression

Mammalian

Promoter

CMV/TO

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-CMV-SpyCas9

Plasmid

#113034

Purpose

AAV vector for expression of SpyCas9 in mammalian cells

Depositor

Dominik Niopek

Publication

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMrfp

Plasmid

#59773

Purpose

Derived from pCM62; ampR, tetR, PBAD - rfp

Depositor

Nathan Hillson

Publication

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti_P2A_Puro

Plasmid

#211364

Purpose

Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

Depositor

Jacques Neefjes

Publication

Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLS13-IL-10

Plasmid

#209128

Purpose

Transient mammalian expression of IL-10

Depositor

Tom de Greef

Publication

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

IL-10 (IL10 Human)

Expression

Mammalian

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGT-Kh2

Plasmid

#122587

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-CBE4max-SpRYc-P2A-EGFP

Plasmid

#208336

Purpose

CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

Depositor

Pranam Chatterjee

Publication

Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

Insert

human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Sc++ (1-1119), SpRY (1111-1368), D10A

Promoter

CAG

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-LOXCATmut

Plasmid

#140085

Purpose

A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

Depositor

Vamsi Mootha

Publication

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Tags

His6

Expression

Bacterial

Mutation

Mutation in H265A and R268A in LOX (used original…

Promoter

T7

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-YFP

Plasmid

#62630

Purpose

mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Publication

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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