We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA-HIRA-polyA

   Plasmid

   #59781

   Purpose

   Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   HIRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmyc-GFP-TNRC6C

   Plasmid

   #42023

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6C (TNRC6C Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. VinculinTS-A50I

   Plasmid

   #111827

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind talin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (A50I)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed alanine 50 to isoleucine

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. nls-Flamindo2

   Plasmid

   #73939

   Purpose

   Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   nls-Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-ZapCY1

   Plasmid

   #36319

   Depositor

   Amy Palmer

   Article

   Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Insert

   ZapCY1 high affinity Zn(II) sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. VinculinTS-I997A

   Plasmid

   #111828

   Purpose

   Vinculin tension sensor with point mutation affecting ability to bind actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed isoleucine 997 to alanine

   Promoter

   CMV

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEQ1180

   Plasmid

   #83805

   Purpose

   express human cytomegalovirus TRS1 with a C-terminal 6X his tag in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Child et al J Virol. 2012 Apr;86(7):3880-9. doi: 10.

   Insert

   TRS1
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. mV-NcVV-LOV_231

   Plasmid

   #58689

   Purpose

   Expresses mVenus-fusion protein of NcVV-LOV domain (Neurospora crassa Vivid)

   Depositor

   Harald Janovjak

   Article

   Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

   Insert

   mV-NcVV-LOV
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RNT2-GFP

    Plasmid

    #17709

    Depositor

    Hal Dietz

    Article

    Mendell et al Mol Cell Biol. 2000 Dec . 20(23):8944-57

    Insert

    rent2 (UPF2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. PE-Cas9

    Plasmid

    #174851

    Purpose

    Express PE-Cas9 in mammalian cells

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    PE-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. myr-palm_mCherry2-mEGFP

    Plasmid

    #127816

    Purpose

    Hetero-dimer mCherry2-mEGFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-miR17

    Plasmid

    #21113

    Depositor

    Joshua Mendell

    Article

    Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    miR-17 (MIR17 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pZZMP122

    Plasmid

    #231756

    Purpose

    Mammalian expression of SPO11-YF

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    SPO11-YF (Spo11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Y138F

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZZMP154

    Plasmid

    #231760

    Purpose

    Mammalian expression of LZ-SPO11

    Depositor

    Scott Keeney

    Article

    Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

    Insert

    LZ-SPO11 (Spo11 Budding Yeast, Mouse)
    

    Use

    Tags

    Flag-LeucineZipper

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-ZapCY2

    Plasmid

    #36320

    Depositor

    Amy Palmer

    Article

    Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Insert

    ZapCY2 high affinity Zn(II) sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    see comment

    Promoter

    CMV

    Available Since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. optGluCl alpha EYFP

    Plasmid

    #15104

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl alpha (glc-1 Nematode)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA

    Promoter

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. p176 Grg-1s Myc His

    Plasmid

    #11065

    Depositor

    Ramesh Shivdasani

    Article

    Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

    Insert

    Grg1-S (Tle1 Mouse)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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