We narrowed to 16,880 results for: Emb;

1. hnRNPA2_LC_nocharge_wR

   Plasmid

   #139119

   Purpose

   express hnRNPA2 LC with no charged residues with R added back

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   remove all K, most D

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUS_LC_charge

   Plasmid

   #139126

   Purpose

   express FUS LC with an hnRNPA2 LC-like charge patterning

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Add R, K, D to make FUS LC have similar charge pa…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-Miro1 (1-177 fused to 562-618)

   Plasmid

   #127627

   Purpose

   First GTPase domain of human Miro1 fused to C-terminus

   Depositor

   Josef Kittler

   Article

   Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

   Insert

   Miro1 (RHOT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   1-177 fused to 562-618

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Oct4deltaPE-53BP1mCherry

   Plasmid

   #141122

   Purpose

   Construct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells

   Depositor

   John Schimenti

   Article

   Bloom et al Genesis. 2020 Aug;58(8):e23368. doi: 10.

   Insert

   Fragment of human 53BP1 (TP53BP1 Human)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NKP91 GFP (1034)

   Plasmid

   #62023

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   Tmem41A (TMEM41A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAV10.F3.loxP

   Plasmid

   #63200

   Purpose

   pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV10.HO5.loxP

   Plasmid

   #63206

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRPML1-G-GECO1.2-ERES

   Plasmid

   #207144

   Purpose

   Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   Mcoln1 (MCOLN1 Human)
   

   Use

   Tags

   ER-Export Signal (ERES) and G-GECO1.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP VAMP7 (1-220)

   Plasmid

   #42316

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 (1-220) (VAMP7 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TPC2(N257A)-mCherry

    Plasmid

    #135186

    Purpose

    Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of asparagine257 to alanine

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TPC2(E643A)-mCherry

    Plasmid

    #135187

    Purpose

    Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Mutation of glutamate 643 to alanine

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pLV-IRF9

    Plasmid

    #71452

    Purpose

    Lentiviral expression of IRF9

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

    Plasmid

    #229812

    Purpose

    A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mCherry
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT-TO-NOD2-EGFP

    Plasmid

    #131207

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

    Insert

    NOD2 (NOD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVpuro-Gcn5

    Plasmid

    #63706

    Purpose

    retroviral expression of mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3/hArf6(WT)-HA

    Plasmid

    #79424

    Purpose

    Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only