We narrowed to 4,893 results for: PIR
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Plasmid#188543PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editingDepositorInsertLbCas12a-RRV
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182VPromoterAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (NRG1 JMD)
Plasmid#188379PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a NRG1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_NRG1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Myb
Plasmid#139826Purposedoxycycline-inducible expression of mouse Myb in mammalian cellsDepositorInsertMyb (Myb Mouse)
UseLentiviralTagsExpressionMammalianMutationP94H and Q619R- Please see depositor comment belo…PromoterTRE-mCMVAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLGB36
Plasmid#135621PurposeSuicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselectionDepositorInsertbfe3
UseBacteroides suicide vector with inducible counter…Tagssignal sequence for periplasmic targetingExpressionMutationPromoterBacteroides promoter regualted by TetR transcript…Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-NFKB1
Plasmid#139822Purposedoxycycline-inducible expression of Human NFKB1 in mammalian cellsDepositorAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
rnLbCas12a-PE2
Plasmid#213746PurposeFor circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cellsDepositorInsertrnLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, H759A, R1138A in LbCas12aPromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Myc-E-Syt2
Plasmid#66834PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
Plasmid#132408Purposeoverexpression in human cellsDepositorInsertdLbaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR600A; H605A; R1243A; H1248APromoterAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LbCpf1-Blast
Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianMutationPromoterEFS-NSAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN456_TALEcharm_Kv2_MmT8x
Plasmid#220835PurposeTALEcharm expression in mammalian cellsDepositorInsertTALEcharm Kv2
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-p160MBP
Plasmid#41DepositorAvailable SinceJuly 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa42_4454
Plasmid#49703DepositorInsertECF42_4454
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST
Plasmid#175020PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutations57a esterase knock out mutantPromoterCMVAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only