We narrowed to 15,903 results for: grna

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PB-GG-OCT4-1-5-PGK-Puro

Plasmid

#102893

Purpose

PiggyBac transposon system construct with 5 concatenated U6 promoter driven transcriptional cassettes for the activation of OCT4. Contains PGK-puro selection cassette.

Depositor

Timo Otonkoski

Publication

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OCT4-gRNAs-1-5-PGK-puro (POU5F1 )

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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human ETV6 gRNA-4

Plasmid

#133404

Purpose

human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

Depositor

Linzhao Cheng

Publication

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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KHC065 pBABE pU6 BRD2 K1-2C

Plasmid

#231712

Purpose

KHC065 (pBABE pU6 BRD2 K1-2C), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC064

Depositor

Alessio Ciulli

Publication

Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

Insert

gRNA for Brd2 and Cas9D10A (BRD2 Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBAtC

Plasmid

#78097

Purpose

Plant expression of Cas9 and gRNA empty backbone; Basta resistance

Depositor

Jin-Soo Kim

Publication

Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

NLS-HA

Expression

Plant

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMpGE_En03

Plasmid

#71535

Purpose

Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

Depositor

Takayuki Kohchi, Sumihare Noji

Publication

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

Plasmid

#149426

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Publication

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-ARHGEF7/B-Pix

Plasmid

#241368

Purpose

Mammalian expression of SpCas9 and gRNA targeting ARHGEF7 (β-Pix)

Depositor

Gaudenz Danuser

Publication

Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

Insert

ARHGEF7 gRNA (ARHGEF7 Human)

Expression

Mammalian

Promoter

U6

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSF278

Plasmid

#167673

Purpose

Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-U6-CB2gRNA-CBh-mCherry

Plasmid

#91948

Purpose

Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

Depositor

Jimok Kim

Publication

Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

Insert

mCherry

Use

AAV

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Publication

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PX458_FOXM1_iso1_2

Plasmid

#135753

Purpose

Encodes gRNA for 3' target of human FOXM1_iso1

Depositor

Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXM1_iso1 gRNA (FOXM1 Human)

Use

CRISPR

Promoter

U6

Available since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_FOXM1_iso1_1

Plasmid

#135752

Purpose

Encodes gRNA for 3' target of human FOXM1_iso1

Depositor

Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXM1_iso1 gRNA (FOXM1 Human)

Use

CRISPR

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC588

Plasmid

#62315

Purpose

sgRNA with 2x MS2 (wt+f6) for yeast cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x MS2 (wt+f6) binding module

Expression

Yeast

Promoter

SNR52

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Publication

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PRKDC gRNA (BRDN0001145772)

Plasmid

#77862

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK1 gRNA (BRDN0001149093)

Plasmid

#77788

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MLKL gRNA (BRDN0001149268)

Plasmid

#77541

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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