We narrowed to 5,031 results for: CAPS

1. pKMW121_WT-Cas9_U6-entry

   Plasmid

   #244818

   Purpose

   Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 (WT)
   

   Use

   CRISPR

   Tags

   3xFLAG-SV40 NLS and Nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   CAG, U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMW493_U6-sgRNA-entry

   Plasmid

   #244823

   Purpose

   Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpyCas9 single guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a_VIM-2

   Plasmid

   #242840

   Purpose

   Expresses His6-TEV-VIM-2 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-2 sequence (UniPROT Q5U7L7)

   Depositor

   Richard Page

   Article

   Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do

   Insert

   Metallo-beta-lactamase VIM-2
   

   Tags

   His6 + Tobacco etch virus (TEV) cleavage site “EN…

   Expression

   Bacterial

   Mutation

   deleted residues 1-26

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a_VIM-20

   Plasmid

   #242842

   Purpose

   Expresses His6-TEV-VIM-20 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-20 sequence (UniPROT A0A344X7M2)

   Depositor

   Richard Page

   Article

   Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do

   Insert

   Metallo-beta-lactamase VIM-20
   

   Tags

   His6 + Tobacco etch virus (TEV) cleavage site “EN…

   Expression

   Bacterial

   Mutation

   deleted residues 1-26

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a_VIM-15

   Plasmid

   #242841

   Purpose

   Expresses His6-TEV-VIM-15 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-15 sequence (UniPROT B4YAJ6)

   Depositor

   Richard Page

   Article

   Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do

   Insert

   Metallo-beta-lactamase VIM-15
   

   Tags

   His6 + Tobacco etch virus (TEV) cleavage site “EN…

   Expression

   Bacterial

   Mutation

   deleted residues 1-26

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a_VIM-31

   Plasmid

   #242843

   Purpose

   Expresses His6-TEV-VIM-31 in bacteria, comprising a His6-tag, the Tobacco etch virus (TEV) cleavage site “ENLYFQ/G", and the VIM-31 sequence (UniPROT I3RJZ3)

   Depositor

   Richard Page

   Article

   Silwal et al J Inorg Biochem. 2025 Oct;271:112975. do

   Insert

   Metallo-beta-lactamase VIM-31
   

   Tags

   His6 + Tobacco etch virus (TEV) cleavage site “EN…

   Expression

   Bacterial

   Mutation

   deleted residues 1-26

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS484_10xHis_MBP_TEV_dSpRY

   Plasmid

   #244832

   Purpose

   Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   dSpRY Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS24_10xHis_MBP_TEV_SpRY-Cas9

   Plasmid

   #244827

   Purpose

   Bacterial expression of SpRY Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpRY Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

   Plasmid

   #244834

   Purpose

   Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   SpG Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP and 2xSV40 NLS

   Expression

   Bacterial

   Mutation

   D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

    Plasmid

    #238184

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGCS-3xHA-TMEM230

    Plasmid

    #225512

    Purpose

    TMEM230 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM230 (TMEM230 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGCS-3xHA-TMEM230triplemut

    Plasmid

    #225513

    Purpose

    TMEM230triplemut expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    TMEM230 (TMEM230 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA -CLCN5-3xFlag

    Plasmid

    #225507

    Purpose

    CLCN5 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN5 (CLCN5 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGCS-3xFlag-ATP11B

    Plasmid

    #225511

    Purpose

    ATP11B expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    ATP11B (ATP11B Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

    Plasmid

    #235591

    Purpose

    Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dot1l (Dot1l Mouse)
    

    Use

    CRISPR

    Mutation

    GS163-164RC

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCA528 huPQBP1

    Plasmid

    #217449

    Purpose

    Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    PQBP1 (PQBP1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. HIV-1-GFP ΔEnv CA E45A

    Plasmid

    #217438

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA E45A

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HIV-1-GFP ΔEnv CA M66I

    Plasmid

    #217440

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA M66I

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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