We narrowed to 176 results for: Araf

1. pUC19-π-EB1_IRES-mCherry

   Plasmid

   #197427

   Purpose

   Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-π-EB1_EF1α-mCherry

   Plasmid

   #197429

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-π-EB1_EF1α-EGFP

   Plasmid

   #197430

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   EGFP, GCN4 leucine zipper, LOV2, and Zdk1

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Duet1-6xHis-TEV-NDP52

   Plasmid

   #190194

   Purpose

   Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Expression

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB1N-LZ-mCherry-LOV2(wt)

   Plasmid

   #107693

   Purpose

   N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB1N-LZ-LOV2(fast)

   Plasmid

   #107690

   Purpose

   N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain (I427V, H519L…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 FLAG FBXL2

   Plasmid

   #236455

   Purpose

   transient overexpression of FBXL2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

   Insert

   FBXL2 (FBXL2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

   Plasmid

   #125712

   Purpose

   Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   eOPN3
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CMV-PdCO-mScarlet

    Plasmid

    #198507

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of CMV promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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