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Showing: 281 - 300 of 471 results

  2. pRS267

    Plasmid
    #122380
    Purpose
    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Insert
    HSP90
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122380-plasmid-map-sequence-id-243382

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  4. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Use
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  7. pATF4-donor

    Plasmid
    #113805
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP
    Depositor
    Kevin White
    Insert
    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs140428747
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113805-plasmid-map-sequence-id-231035

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  9. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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  10. pYZ673

    Plasmid
    #132953
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    FnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    adh1
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    132953-plasmid-map-sequence-id-310101

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  13. pYZ675

    Plasmid
    #132954
    Purpose
    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132954-plasmid-map-sequence-id-307989

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  14. pYZ694

    Plasmid
    #132956
    Purpose
    crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe
    Depositor
    Jef Boeke
    Article
    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578
    Insert
    dFnCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    adh1
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    132956-plasmid-map-sequence-id-307975

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  18. pEHF-donor

    Plasmid
    #113817
    Purpose
    CRISPR donor plasmid to tag human transcription factor EHF with GFP
    Depositor
    Kevin White
    Insert
    EHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs3818514, rs11032804, hg38:11:34659087:G>A
    Promoter
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113817-plasmid-map-sequence-id-230183

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  19. CMYA5tnT/pDEST26-N-FLAG

    Plasmid
    #98131
    Purpose
    Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Rita Shiang
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    rs10043986 (NM_153610.4:c.12188C>T)
    Promoter
    CMV
    Available since
    Aug. 3, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98131-plasmid-map-sequence-id-190024

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Showing: 281 - 300 of 471 results