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Showing: 421 - 450 of 25489 results

  1. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid
    #182359
    Purpose
    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Insert
    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Promoter Ultra Pan Neuronal
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182359-plasmid-map-sequence-id-361684

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  2. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187949
    Purpose
    SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SMASh-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187949-plasmid-map-sequence-id-373500

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  3. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid
    #182355
    Purpose
    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182355-plasmid-map-sequence-id-361664

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  4. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid
    #182347
    Purpose
    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182347-plasmid-map-sequence-id-361781

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  6. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid
    #182349
    Purpose
    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182349-plasmid-map-sequence-id-361661

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  9. pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

    Plasmid
    #182354
    Purpose
    Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182354-plasmid-map-sequence-id-361685

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  10. pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

    Plasmid
    #182356
    Purpose
    Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)
    Depositor
    Christian Froekjaer-Jensen
    Article
    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    182356-plasmid-map-sequence-id-361657

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  12. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

    Plasmid
    #214140
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214140-plasmid-map-sequence-id-431241

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  13. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid
    #214139
    Purpose
    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214139-plasmid-map-sequence-id-431260

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  18. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid
    #232929
    Purpose
    Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    OsTir1(F74G)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    changed Phenylalanine 74 to Glycine
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232929-plasmid-map-sequence-id-466497

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  20. pAc5.1B-lambdaN-HA-DmAUB_A

    Plasmid
    #145851
    Purpose
    Insect Expression of DmAUB
    Depositor
    Elisa Izaurralde
    Insert
    DmAUB (aub Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Two silent mutations compared to the sequence giv…
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145851-plasmid-map-sequence-id-288296

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  21. pattB-aubergine-WT-gf

    Plasmid
    #69447
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid to expression Aubergine
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Use
    Tags
    NA
    Expression
    Insect
    Mutation
    many SNPs exist in the promoter region of aubergi…
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69447-plasmid-map-sequence-id-122548

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  22. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Use
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69448-plasmid-map-sequence-id-122550

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  24. pWR63(YUM1)

    Plasmid
    #203336
    Purpose
    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203336-plasmid-map-sequence-id-417292

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  25. pWR63

    Plasmid
    #203331
    Purpose
    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    203331-plasmid-map-sequence-id-409751

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  26. pWR76

    Plasmid
    #202776
    Purpose
    episomal fluorescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202776-plasmid-map-sequence-id-417311

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  27. pWR23

    Plasmid
    #202770
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202770-plasmid-map-sequence-id-417278

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  28. pWR10

    Plasmid
    #202710
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202710-plasmid-map-sequence-id-417297

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  29. pWR9

    Plasmid
    #202645
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202645-plasmid-map-sequence-id-409735

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  30. pWR54

    Plasmid
    #203330
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    203330-plasmid-map-sequence-id-417329

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Showing: 421 - 450 of 25489 results