-
Plasmid#135443PurposeMamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFPDepositorInsertTOM20-HaloTag7 and EGFP
UseTagsTOM20ExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B_Halo_T2A_EGFP
Plasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
UseTagsH2BExpressionMammalianMutationPromoterCMV/TetOAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
UseTagsHaloTagExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HaloKu70 sgRNA
Plasmid#207583PurposesgRNA for the insert of the HaloTag at the endogenous loci of Ku70.DepositorInsertGAGCAGTAGCCAACATGTCA
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Lifeact_Halo_T2A_EGFP
Plasmid#135445PurposeMamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFPDepositorInsertLifact-HaloTag7 and EGFP
UseTagsLifactExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-halo-HA-p2a-neoR
Plasmid#161977Purposehomologous donor template for endogenous taggingDepositorInsertGAlinker-HA-halo-HA-p2a-neoR
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-puroR-p2a-HA-halo
Plasmid#161979Purposehomologous donor template for endogenous taggingDepositorInsertpuroR-p2a-HA-halo-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
Plasmid#197066PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBPDepositorInsertEncapsulin
UseCRISPRTagsFKBP and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorInsertgenomic sequence AP1M1 locus (AP1M1 Human)
UseCRISPRTagsHalo, ALFAExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQ
Plasmid#166948PurposeLentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationPromotereF1aAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHalo∆
Plasmid#194659PurposeProtein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteriaDepositorInsertcpHalo∆
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
UbC-Halo-SFPQ
Plasmid#166946PurposeLentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationPromoterUbCAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cdk9-Halo donor
Plasmid#113113PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequenceDepositorInsertCdk9 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Erk2 donor
Plasmid#113104PurposeDonor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequenceDepositorInsertN terminal Erk2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Id2-Halo donor
Plasmid#113116PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequenceDepositorInsertId2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-HaloSFPQY527A
Plasmid#166949PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
UbC-Halo-SFPQY527A
Plasmid#166947PurposeLentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoterDepositorInsertSFPQ-Y527A (SFPQ Human)
UseLentiviralTagsHaloExpressionMutationSFPQ-Y527APromoterUbCAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C DCC -Halo
Plasmid#195150Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove coi…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT--Halo
Plasmid#195152Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationmutations to make si RNA resistant and remove act…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3C CC 411-615
Plasmid#193936PurposeMammalian expression of Halo-tagged Kif 3C CC 411-615DepositorInsertKif 3C (Kif3c Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3A CC 419-589
Plasmid#193926PurposeMammalian expression of Halo-tagged Kif 3A CC 419-589DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3C DD 616-796
Plasmid#193938PurposeMammalian expression of Halo-tagged Kif 3C DD 616-796DepositorInsertKif 3C (Kif3c Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B CC 391-592
Plasmid#193931PurposeMammalian expression of Halo-tagged Kif 3B CC 391-592DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3B DD 593-747
Plasmid#193933PurposeMammalian expression of Halo-tagged Kif 3B DD 593-747DepositorInsertKif 3B (Kif3b Mouse)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-KIF3A DD 590-702
Plasmid#193928PurposeMammalian expression of Halo-tagged Kif 3A DD 590-702DepositorInsertKif 3A (KIF3A Human)
UseTagsHaloExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-DNAPKcs HRD
Plasmid#207088PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2 HRD
Plasmid#207544PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cellsDepositorInsertHaloTag flanked by human WIPI2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG9A HRD
Plasmid#203222PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cellsDepositorInsertHaloTag flanked by human ATG9 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
NBS1-Halo HRD
Plasmid#207084PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous NBS1 locus.DepositorInsertHaloTag followed by a LoxP-PuroR cassette flanked by human NBS1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG16L1 HRD
Plasmid#207537PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Rev7-Halo HRD
Plasmid#207079PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG2A HRD
Plasmid#207540PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cellsDepositorInsertHaloTag flanked by human ATG2A locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 HRD
Plasmid#207086PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATM HRD
Plasmid#207085PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human ATM locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
ATM-Halo HRD
Plasmid#207082PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-53BP1 HRD
Plasmid#207087PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD3 HRD
Plasmid#207077PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only