We narrowed to 4,367 results for: 424

1. pET-3c-rhVTN-NC

   Plasmid

   #30226

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with N and C terminus cleavages (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478, 20-62

   Available Since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

   Plasmid

   #212675

   Purpose

   Express tagged hRb phosphosite mutant

   Depositor

   Yumi Konagaya, Tobias Meyer

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   15 CDK phospho-sites are mutated to alanines, and…

   Promoter

   TRE

   Available Since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-3c-rhVTN-C

   Plasmid

   #30224

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin with C terminus cleavage (VTN Human)
   

   Expression

   Bacterial

   Mutation

   deleted amino acids: 399-478

   Available Since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. LSB-hsa-miR-425-3p

   Plasmid

   #103521

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-425-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-425-3p target (MIR424 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLHCX-mPM20D1-flag

   Plasmid

   #84566

   Purpose

   mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

   Depositor

   Bruce Spiegelman

   Article

   Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

   Insert

   peptidase M20 domain containing 1 (Pm20d1 Mouse)
   

   Use

   Retroviral

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sirius-H2B-6

   Plasmid

   #55225

   Purpose

   Localization: Nucleus/Histones, Excitation: 355, Emission: 424

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Tags

   Sirius

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pYeDP60-hATP13A2-WT

   Plasmid

   #171377

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Tags

   BAD tag

   Expression

   Yeast

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti HsATP13A2 D508N

   Plasmid

   #171820

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   WT, D962N

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti HsATP13A2 WT

   Plasmid

   #171485

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   D508N , D962N

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti HsATP13A2 D962N

    Plasmid

    #171821

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Mutation

    WT, D508N

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDam-Myc-HP1

    Plasmid

    #59221

    Purpose

    expresses Dam-myc-HP1

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Insert

    HP1 (Su(var)205 Fly)
    

    Use

    Damid

    Tags

    Dam and myc

    Expression

    Insect

    Promoter

    Drosophila heat shock promoter

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. miR5 Mm ATP13A2

    Plasmid

    #171822

    Purpose

    transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    ATP13A2 (Atp13a2 Mouse)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. miR3 Mm ATP13A2

    Plasmid

    #171771

    Purpose

    transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    ATP13A2 (Atp13a2 Mouse)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sirius-H2B-C-10

    Plasmid

    #55226

    Purpose

    Localization: Nucleus/Histones, Excitation: 355, Emission: 424

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Tags

    Sirius

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. NanoLuc-CECR2(BD) Fusion Vector

    Plasmid

    #237203

    Purpose

    Express NanoLuc(R)-CECR2(BD) Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    CECR2 (CECR2 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Mutation

    Bromodomain only (residue 424-543)

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pRK Smad3C Flag

    Plasmid

    #14830

    Depositor

    Rik Derynck

    Article

    Zhang et al Curr Biol. 1997 Apr 1. 7(4):270-6.

    Insert

    Smad3 C (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    amino acids 199-424

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

19. Sirius-N1

    Plasmid

    #54506

    Purpose

    Localization: N1 Cloning Vector, Excitation: 355, Emission: 424

    Depositor

    Michael Davidson

    Article

    Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

    Type

    Empty backbone

    Tags

    Sirius

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. -962 human cyclin D1 promoter EtsC site mutant pGL3Basic

    Plasmid

    #32731

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Mutation

    -424 EtsC site TTCCT deletion

    Promoter

    -962 CCND1 promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only