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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215905

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup-C

Plasmid

#90022

Purpose

To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

C-terminal region alone

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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ZsGreen1-cMYC/pLVX-Puromycin

Plasmid

#180278

Purpose

Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

ZsGreen1-cMYC fusion protein MYC (MYC Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Expression

Mutation

∆DLYC

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p7612 MSCV-P C-FlagHA 16E7 E10K

Plasmid

#163303

Purpose

Expresses HPV16 E7 E10K

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 E10K

Use

Retroviral

Tags

FlagHA

Expression

Mutation

E10K

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Myc-ASC

Plasmid

#73952

Purpose

Express c-Myc-tagged human ASC in mammalian cells

Depositor

Christian Stehlik

Article

Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSecTaq2-hGas6-Myc-6xHis

Plasmid

#226523

Purpose

Expresses TAM Receptor Ligand human GAS6

Depositor

Raymond Birge, Sergei Kotenko

Article

Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

Insert

Human GAS6 (GAS6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Puro

Plasmid

#169220

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Puro

Plasmid

#169228

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Hygro

Plasmid

#169225

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Hygro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-CCR5

Plasmid

#98948

Purpose

Mammalian expression plasmid for N-terminal myc-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYC

Plasmid

#167715

Purpose

Expresses MYC in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYC (MYC Human)

Use

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP(C3)-Nup153-C (880-1475)

Plasmid

#64317

Purpose

GFP-tagged mammalian expression of C-terminal domain of Nup153

Depositor

Birthe Fahrenkrog

Article

Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

Insert

Nup153 (NUP153 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C-terminal subunit (880-1475)

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
p8130 MSCV GFP Puro C-FlagHA

Plasmid

#184521

Purpose

Expression of GFP

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

GFP

Use

Retroviral

Tags

Flag, HA

Expression

Mutation

Promoter

MSCV LTR

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

Plasmid

#122959

Purpose

Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Insert

NOTCH2NL-delta C terminus (NOTCH2NLB Human)

Use

Lentiviral

Tags

6xmyc tag

Expression

Mutation

deletion of C terminus from full length NOTCH2NLB

Promoter

CAG promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-H450A-C-TAP

Plasmid

#83478

Purpose

Mammalian expression of the dehydrogenase-deficient mutant DLD-H450A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

H450A

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pPHAGE_DLD-S456A-C-TAP

Plasmid

#83479

Purpose

Mammalian expression of the proteolysis-deficient mutant DLD-S456A.

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

DLD (DLD Human)

Use

Lentiviral

Tags

TAP

Expression

Mammalian

Mutation

S456A

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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