165,075 results

1. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

   Plasmid

   #235576

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. CuO-LacI-NLS–SNAPf

   Plasmid

   #227368

   Purpose

   Lentiviral expression of LacI–SNAP

   Depositor

   David Pellman

   Article

   Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

   Insert

   LacI NLS SNAPf
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-1 shRev-Erbalpha

   Plasmid

   #22747

   Depositor

   Bruce Spiegelman

   Article

   Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

   Insert

   shRev-Erbalpha (Nr1d1 Mouse)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAW12.lentiguide.GFP

   Plasmid

   #104374

   Purpose

   GFP vector for cloning in guides

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4-VP16

   Plasmid

   #71728

   Purpose

   plasmid expressing fusion construct of Gal4 DBD and VP16 AD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD(1-147) (GAL4 Budding Yeast)
   

   Tags

   VP16 AD (413-490)

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1_His-TEV-EGFP

   Plasmid

   #223723

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   EGFP, not monomeric (has A207)
   

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-GFP-IRF3

   Plasmid

   #127663

   Purpose

   Over-express EGFP-IRF3

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   IRF3 (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubiquitin WT

    Plasmid

    #12647

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubiquitin - wild type (UBC Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2593

    Plasmid

    #142336

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPAmCherry1-N1

    Plasmid

    #31928

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    cmv

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYFAC-CH2-ubi-Y-pyrG

    Plasmid

    #221131

    Purpose

    Expression of heterologous genes under alcohol induction optimised with ubi-Y-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

    Type

    Empty backbone

    Use

    Synthetic Biology; Expression in aspergillus and …

    Promoter

    PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 Connexin43-GFP-APEX2

    Plasmid

    #49385

    Purpose

    Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Connexin43 - EGFP - APEX2
    

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available Since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB_TRE3G_scFV-sfGFP-Kmt5cFL

    Plasmid

    #235581

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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