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Showing: 5961 - 6000 of 8360 results

  1. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Use
    Tags
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  2. pDONR221-CTSB

    Plasmid
    #158443
    Purpose
    Gateway Cloning compatible entry vector for the human CTSB gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158443-plasmid-map-sequence-id-308885

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  3. TAF1

    Plasmid
    #39117
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  6. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61K
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83180-plasmid-map-sequence-id-158143

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  7. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    SRF (SRF Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. dSaCas9-KRAB

    Plasmid
    #205250
    Purpose
    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.
    Depositor
    Rasmus Bak
    Insert
    dSaCas9-KRAB
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205250-plasmid-map-sequence-id-409107

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  9. pLenti-cytoMetGFP

    Plasmid
    #37561
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only contains cytoplasmic domain amino acids K956…
    Promoter
    CMV
    Available since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    37561-plasmid-map-sequence-id-47323

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  10. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Gavin Thomas, Emmanuele Severi
    Insert
    F5-flanked tetracycline resistance cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Promoter
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  12. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Eleanor Chen
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83966-plasmid-map-sequence-id-160487

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  13. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

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  14. pFRT-TODestFLAGHA_HuB

    Plasmid
    #65755
    Purpose
    expresses FLAGHA-tagged HuB in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL2 (ELAVL2 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    65755-plasmid-map-sequence-id-121638

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  15. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Use
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

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  16. pAG416-GAL-TDP-43

    Plasmid
    #181709
    Purpose
    Galactose inducible expression of TDP-43
    Depositor
    Alejandro Chavez
    Insert
    TDP-43 (TARDBP Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181709-plasmid-map-sequence-id-430952

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  17. isoform PKC Iota (JCE597)

    Plasmid
    #182030
    Purpose
    yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Iota (Prkci Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182030-plasmid-map-sequence-id-361960

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  19. pIVT-myc-T58A-S62D

    Plasmid
    #138540
    Purpose
    For making myc-T58A-S62D RNA
    Depositor
    Carmen Williams
    Insert
    Myelocytomatosis oncogene T58A S62D (Myc Mouse)
    Use
    In vitro transcription
    Tags
    Expression
    Mutation
    Threonine 58 changed to alanine and serine 62 cha…
    Promoter
    T7
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    138540-plasmid-map-sequence-id-291746

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  20. pLenti-EGFP-hChibby1

    Plasmid
    #89770
    Purpose
    Labels mother centrioles/basal bodies
    Depositor
    Ken-Ichi Takemaru
    Insert
    Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    EF1alpha
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    89770-plasmid-map-sequence-id-193659

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  21. pLEX307-CTSB-G418

    Plasmid
    #158465
    Purpose
    Constitutive lentiviral expression of human CTSB.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158465-plasmid-map-sequence-id-308157

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  22. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. tet-pLKO.puro_shGAC

    Plasmid
    #110423
    Purpose
    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110423-plasmid-map-sequence-id-213314

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  24. C14orf169

    Plasmid
    #39114
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    C14orf169 (C14orf169 Human)
    Use
    Tags
    Tev His Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Use
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  26. pLEX307-CTSB-blast

    Plasmid
    #158464
    Purpose
    Constitutive lentiviral expression of human CTSB.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158464-plasmid-map-sequence-id-308167

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  27. GOT1

    Plasmid
    #39022
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GOT1 (GOT1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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  29. Hs.HRAS Q61L

    Plasmid
    #83186
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Q61L
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83186-plasmid-map-sequence-id-158149

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  30. MAPK11

    Plasmid
    #39005
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK11 (MAPK11 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. CAMK2B

    Plasmid
    #38873
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2B (Camk2b Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. CBLB

    Plasmid
    #39056
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBLB (CBLB Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pLenti-tkd2MetGFP

    Plasmid
    #37562
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only contains cytoplasmic domain amino acids L115…
    Promoter
    CMV
    Available since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    37562-plasmid-map-sequence-id-47324

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  36. HOXA2 (murine) HIS-tag pET

    Plasmid
    #8557
    Depositor
    Corey Largman
    Insert
    HOXA2 (Hoxa2 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    contains 5 additional amino acids from 5' un…
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  37. KAT2A

    Plasmid
    #38914
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2A (KAT2A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pBabe-Kras G15A

    Plasmid
    #58898
    Purpose
    Expresses human Kras with a G15A mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G15A mutation
    Promoter
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. GPX1

    Plasmid
    #38864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPX1 (Gpx1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys49 -> Gly
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. JMJD2C

    Plasmid
    #39070
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD2C (KDM4C Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5961 - 6000 of 8360 results