We narrowed to 13,673 results for: Cre

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pGWB540

Plasmid

#74874

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-634)

Plasmid

#25718

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-634) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

Plasmid

#196885

Purpose

Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA

Plasmid

#113687

Purpose

SaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9 (NEWENTRY )

Use

AAV and CRISPR

Tags

Flag, HAx2, KASH, and NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCHW111118

Plasmid

#222461

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -0

Plasmid

#173889

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

Plasmid

#173890

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1

Plasmid

#139860

Purpose

Lentiviral expression of HA-tagged human UFL1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1 (UFL1 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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p11L1w

Plasmid

#37330

Depositor

John Schiller

Article

Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

Insert

HPV11 L1

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM22082_sfGFP1-10

Plasmid

#153515

Purpose

E.coli expression plasmid for the production of GFP1-10

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

sfGFP1-10

Expression

Bacterial

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-ATAD2

Plasmid

#65370

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ATAD2 (ATAD2 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A-V5

Plasmid

#35927

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

April 20, 2012

Availability

Academic Institutions and Nonprofits only

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Str-Ii_neomycin

Plasmid

#65312

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its cytoplasmid part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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HF-PX459 (V2)

Plasmid

#118632

Purpose

Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

Depositor

Mike McGrew

Article

Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

Insert

hSpCas9-HF1-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Asparagine 497 to Alanine, Arginine 661 to Alanin…

Promoter

Cbh

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Article

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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