We narrowed to 10,893 results for: ENA;

1. pET28-sfGFP[N150TAA]

   Plasmid

   #164581

   Purpose

   superfolder GFP with N150TAA mutation

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Insert

   sfGFP-150TAA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N150TAA Mutation

   Promoter

   T7

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-sh-mSOX9-5

   Plasmid

   #40646

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-5 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pPUR-mU6-sgRNA-2XPP7

   Plasmid

   #194504

   Purpose

   Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

   Depositor

   Chunqing Song

   Article

   Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSF107mT-GATEWAY-3'-FLAG

   Plasmid

   #67619

   Purpose

   A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

   Depositor

   Todd Stukenberg

   Article

   Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

   Type

   Empty backbone

   Use

   In vitro transcription or translation off of an s…

   Tags

   C-terminal Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available Since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-C1_SEPT9 i1

   Plasmid

   #71622

   Purpose

   mammalian expression of human SEPT9 with an mCherry fusion

   Depositor

   Elias Spiliotis

   Article

   Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1159

   Plasmid

   #119250

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF806-shRen.713

   Plasmid

   #186712

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   shRen.713
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVSVΔG-eGFP-linker

    Plasmid

    #231707

    Purpose

    Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV antigenome
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    ΔG (VSV glycoprotein removed)

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. M13cp-dg3 hp

    Plasmid

    #218095

    Purpose

    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII

    Depositor

    Kevin Esvelt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-HSPA8

    Plasmid

    #121161

    Purpose

    Mammalian expression of GFP tagged HSPA8

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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