We narrowed to 31,552 results for: Eng;

1. pET28 mePOU-POU

   Plasmid

   #206392

   Purpose

   Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. iCAP-BI-49-KanR

   Plasmid

   #203534

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI49 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. iCAP-BI-65-KanR

   Plasmid

   #203536

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   AAV-BI65 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-15b-LanCL1-H219A

   Plasmid

   #154190

   Purpose

   To express LanCL1-H219A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His219->Ala219

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-15b-LanCL1-C322A

   Plasmid

   #154191

   Purpose

   To express LanCL1-C322A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Cys322->Ala322

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL-p2x-LanCL2

   Plasmid

   #154192

   Purpose

   To express MBP-LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Maltose binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-TTBG

   Plasmid

   #115232

   Purpose

   Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-TTBL

    Plasmid

    #115233

    Purpose

    Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLKO.1-Cd155 shRNA2

    Plasmid

    #166488

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLKO.1-Cd155 shRNA1

    Plasmid

    #166487

    Purpose

    Mouse Cd155 RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Cd155 (Pvr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-N1-MOB1

    Plasmid

    #166477

    Purpose

    Expresses fusion of mCherry and MOB1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, MOB1 (MOB1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PatoM-OG241

    Plasmid

    #72842

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoM

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEXP5-NT-7th R

    Plasmid

    #42573

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated calmodulin gene
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_iPE-C_P2A_Csy4

    Plasmid

    #232428

    Purpose

    Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    iPE-C–P2A–Csy4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHE111-3xFlag-HSP70

    Plasmid

    #236055

    Purpose

    Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    HSP70 (HSPA1A Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE-FGFR3__TACC3

    Plasmid

    #116818

    Purpose

    Lentiviral expression of FGFR3__TACC3 fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3__TACC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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