We narrowed to 8,325 results for: Set

1. eSpCas9(1.1)_No_FLAG_ATP1A1-G7

   Plasmid

   #173203

   Purpose

   Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. JP705: pMVP/SB/Neo-DEST

   Plasmid

   #121866

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ATP1A1_G8_Dual_sgRNA

   Plasmid

   #178104

   Purpose

   Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G8 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. KK001: pMVP/SB/Hygro-DEST

   Plasmid

   #121867

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKI-cjFOXL2-coemiRFP670-PGK-Puro

   Plasmid

   #186168

   Purpose

   Knock-in gene targeting in marmoset cells at FOXL2 locus

   Depositor

   Sho Yoshimatsu

   Article

   Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

   Insert

   emiRFP670
   

   Use

   Marmoset targeting

   Tags

   Expression

   Mutation

   human codon-optimized

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LN-entry

   Plasmid

   #128012

   Purpose

   Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag
   

   Use

   Lamdan entry vector

   Tags

   LN-3xFlag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1352wUV2 F3bbs kan NNNTGGGCGk

   Plasmid

   #18756

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Promoter

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-KD-mAbl

   Plasmid

   #69028

   Purpose

   Retorviral vector encoding Kinase Defective mouse c-Abl

   Depositor

   Jean Wang

   Article

   Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

   Insert

   mouse c-Abl Type VI - kinase defective
   

   Use

   Retroviral

   Tags

   FLAG tag inserted at Internal SalI site (2934 bp)

   Expression

   Mammalian

   Mutation

   The KD (kinase defective) mutation is at Lysine 2…

   Promoter

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMOD_B2121

    Plasmid

    #91065

    Purpose

    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    GmUbi

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1352wUV2 F1bbs kan GCGTGGNNN

    Plasmid

    #18753

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Promoter

    Available since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOD_C2516

    Plasmid

    #91084

    Purpose

    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    At7SL

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DCT.VV.Orf207

    Plasmid

    #12895

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf207
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

14. aav-U6-broccoli-hsp68-mCherry-MCS-pA

    Plasmid

    #195411

    Purpose

    AAV9 based enhancer activity assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JT504: pMVP/SB/eGFP-DEST

    Plasmid

    #121861

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JT601: pMVP/SB/mCherry-DEST

    Plasmid

    #121862

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DCT.VV.Orf166

    Plasmid

    #12854

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf166
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

18. DCT.VV.Orf212

    Plasmid

    #12900

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf212
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pMOD_B2519

    Plasmid

    #91076

    Purpose

    Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU3

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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