We narrowed to 59,336 results for: cloning

1. pENTR221-WNT1

   Plasmid

   #79499

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WNT1 (WNT1 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-FRB(KTF)-HA no-pA

   Plasmid

   #82596

   Purpose

   3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRB-HA (no pA)
   

   Mutation

   W2101F

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4440

   Plasmid

   #1654

   Depositor

   Andrew Fire

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, T7p, lacZN, OriF1>>, OriF1<<

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZP06C

   Plasmid

   #232322

   Purpose

   pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

   Depositor

   Chenggang Wu

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   rpsJ

   Available Since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. OTUB1

   Plasmid

   #25149

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   otub1.040.271 (OTUB1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acid residues 40-271

   Available Since

   Aug. 6, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCMV14_Fibrillarin

   Plasmid

   #73061

   Purpose

   expression clone for human Fibrillarin with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. plenti guide Thy1.1

   Plasmid

   #128063

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCROP-PuroR-P2A-GFP-U6

   Plasmid

   #200029

   Purpose

   Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   PuroR-P2A-GFP

   Expression

   Mammalian

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZP06

   Plasmid

   #232320

   Purpose

   pZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.

   Depositor

   Chenggang Wu

   Insert

   Pfdx-E
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PPT1

    Plasmid

    #25205

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PPT1_02 (PPT1 Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. NAT1

    Plasmid

    #25511

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NAT1 (NAT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. PRDM1

    Plasmid

    #25249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM1_04 (PRDM1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pDD362

    Plasmid

    #137023

    Purpose

    SapTrap HaloTag-GLO donor

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    C. elegans GLO-optimized Halo Tag
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized Halo Tag

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. UBC80

    Plasmid

    #25267

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc80.229.304 (TSG101 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. RALGDS

    Plasmid

    #25331

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RALGDS:N793-F914 (RALGDS Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids N793-F914

    Available Since

    Sept. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. Pf-ClpP

    Plasmid

    #25591

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-ClpP
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. SUV39H2

    Plasmid

    #25115

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SUV39H2_04 (SUV39H2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. UBC32

    Plasmid

    #25213

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc32.001.158 (UBE2K Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. RNF168

    Plasmid

    #25298

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    rnf168.0001.0113 (RNF168 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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