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p3E-mScarlet

Plasmid

#140875

Purpose

Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-iRFPBb

Plasmid

#118237

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven iRFP

Expression

Bacterial

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0031-mCherryBb

Plasmid

#118240

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0031 promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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myc-hULK2

Plasmid

#31966

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Lmajor_gp63_10_0460_WT

Plasmid

#171640

Purpose

Expression of wildtype L. major glcyoprotein-63 from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

Insert

LmjF.10.0460

Tags

Myc-His

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pYpet-C1

Plasmid

#22780

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Ypet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
myc-mULK1M92A

Plasmid

#31962

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

myc

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
pCyPet-C1

Plasmid

#22779

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

CyPet

Expression

Mammalian

Mutation

Control for intermolecular Rac1 biosensor FRET. S…

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only
XW09

Plasmid

#65833

Purpose

Punc-4c-QS-SL2-mcherry3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ906

Plasmid

#44472

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, unc-119, [4-3] Gateway

Use

Gateway destination vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 mFz8CRD-IgG

Plasmid

#16689

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Frizzled-8 (Fzd8 Mouse)

Tags

IgG

Expression

Mammalian

Mutation

CRD domain of mFz8

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCFJ1662

Plasmid

#51482

Purpose

miniMos transposable element with HygroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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Casp1

Plasmid

#98849

Purpose

Expresses murine Casp1 in mammalian cells

Depositor

Peter Keyel

Article

Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

Insert

Casp1 (Casp1 Mouse)

Tags

No fusion proteins or tags

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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