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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 31,241 results for: cmv

Showing: 6041 - 6080 of 31241 results

  1. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid
    #221566
    Purpose
    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 16nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221566-plasmid-map-sequence-id-437904

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  2. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

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  3. pCiGSΔΨ

    Plasmid
    #108312
    Purpose
    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    David Russell
    Article
    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.
    Insert
    gag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    pCiGSΔΨ is a newer version of the pCiGS Gag expre…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108312-plasmid-map-sequence-id-211440

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  8. iRFP-H2A

    Plasmid
    #158691
    Purpose
    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Insert
    iRFP670
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158691-plasmid-map-sequence-id-311047

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  9. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  12. pNLF1W

    Plasmid
    #136404
    Purpose
    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    136404-plasmid-map-sequence-id-271260

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  14. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69024-plasmid-map-sequence-id-127982

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  18. pAAV-HyPer-DAAO-NES

    Plasmid
    #119164
    Purpose
    Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.
    Depositor
    Thomas Michel
    Article
    Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1
    Insert
    HyPer-DAAO-NES
    Use
    AAV
    Tags
    NES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119164-plasmid-map-sequence-id-232464

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  19. Human a4 nicotinic subunit crystallization construct

    Plasmid
    #85841
    Purpose
    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85841-plasmid-map-sequence-id-171121

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  20. Human b2 nicotinic subunit crystallization construct

    Plasmid
    #85842
    Purpose
    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct
    Depositor
    Ryan Hibbs
    Article
    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do
    Insert
    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    C-terminal StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    85842-plasmid-map-sequence-id-171117

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  27. pAcCas12nHS_RV6_empty

    Plasmid
    #203811
    Purpose
    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.
    Depositor
    Quanjiang Ji
    Article
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Insert
    AcCas12nHS_RV6
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203811-plasmid-map-sequence-id-405075

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  28. Cx26-msfGFP

    Plasmid
    #69016
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx26 (Gjb2 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69016-plasmid-map-sequence-id-124712

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  34. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  36. Cx43K258stop-msfGFP

    Plasmid
    #69023
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Mutation
    delete codons 258-381 and fuse in frame to monome…
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69023-plasmid-map-sequence-id-124708

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  37. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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  40. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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Showing: 6041 - 6080 of 31241 results