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Showing: 7551 - 7600 of 12238 results

  1. pCfb13211

    Plasmid
    #219886
    Purpose
    The base plasmid of TUNEYALI for TF21
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF21 (YALI1_E20635g) and homologous arm matching TF21
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219886-plasmid-map-sequence-id-435395

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  2. pCfb13215

    Plasmid
    #219890
    Purpose
    The base plasmid of TUNEYALI for TF25
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF25 (YALI1_B18134g) and homologous arm matching TF25
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219890-plasmid-map-sequence-id-435394

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  3. pCfb13221

    Plasmid
    #219896
    Purpose
    The base plasmid of TUNEYALI for TF31
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF31 (YALI1_D18727g) and homologous arm matching TF31
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219896-plasmid-map-sequence-id-435390

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  4. pCfb13235

    Plasmid
    #219908
    Purpose
    The base plasmid of TUNEYALI for TF45
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF45 ( YALI1_B23373g) and homologous arm matching TF45
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219908-plasmid-map-sequence-id-435389

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  5. pCfb13212

    Plasmid
    #219887
    Purpose
    The base plasmid of TUNEYALI for TF22
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF22 (YALI1_C25288g) and homologous arm matching TF22
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219887-plasmid-map-sequence-id-435385

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  6. pCfb13202

    Plasmid
    #219877
    Purpose
    The base plasmid of TUNEYALI forTF12
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF12 (YALI1_C06870g) and homologous arm matching TF12
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219877-plasmid-map-sequence-id-435382

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  7. pCfb13234

    Plasmid
    #219907
    Purpose
    The base plasmid of TUNEYALI for TF44
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF44 (YALI1_B22561g) and homologous arm matching TF44
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219907-plasmid-map-sequence-id-435381

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  8. pCfb13219

    Plasmid
    #219894
    Purpose
    The base plasmid of TUNEYALI for TF29
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF29 (YALI1_C09133g) and homologous arm matching TF29
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219894-plasmid-map-sequence-id-435380

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  9. pCfb13248

    Plasmid
    #219919
    Purpose
    The base plasmid of TUNEYALI for TF58
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219919-plasmid-map-sequence-id-435379

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  10. pKP2188

    Plasmid
    #167642
    Purpose
    Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V16C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    FepA with V16C, I14P mutations. (They are numbere…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167642-plasmid-map-sequence-id-337262

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  11. pFGL1009

    Plasmid
    #119080
    Purpose
    Introducing ectopic integration at the defined fungal ILV2-locus, based on SRR (Sulfonylurea Resistance Reconstitution).
    Depositor
    Naweed Naqvi
    Type
    Empty backbone
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    119080-plasmid-map-sequence-id-232227

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  12. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. p15TV-L

    Plasmid
    #26093
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Sept. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    26093-plasmid-map-sequence-id-52817

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  14. Col1A2:TFPnls-T2a-mErt-Cre-Ert

    Plasmid
    #111151
    Purpose
    Fibroblasts specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    wt
    Promoter
    Col1A2
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111151-plasmid-map-sequence-id-211534

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  15. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  16. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Mutation
    Promoter
    pRhaBAD
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  17. Prrx1:TFPnls-T2a-Cre-Ert

    Plasmid
    #111150
    Purpose
    Limb connective tissue specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-Cre-Ert
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    wt
    Promoter
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111150-plasmid-map-sequence-id-211530

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  18. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac0
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

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  19. pOPINEneo-3C-GFP

    Plasmid
    #53534
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    GFP-His8
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53534-plasmid-map-sequence-id-83411

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  20. pLexA::nud-1

    Plasmid
    #11339
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  21. pLexA::lis-1

    Plasmid
    #11338
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  22. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid
    #54489
    Purpose
    PCR based C-terminal SRM tagging in yeast
    Depositor
    Eri Sakata
    Insert
    SRM (sortase-recognition motif)
    Use
    Yeast genome targeting
    Tags
    kanMX6
    Expression
    Mutation
    Promoter
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. dClover2-CytERM-N-17

    Plasmid
    #56308
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
    Depositor
    Michael Davidson
    Insert
    CytERM
    Use
    Tags
    dClover2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56308-plasmid-map-sequence-id-178445

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  24. pN23-LIC-FLAG-STREP

    Plasmid
    #182508
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and STREP
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    STREP-FLAG-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182508-plasmid-map-sequence-id-361393

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  25. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  26. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  28. TETv4

    Plasmid
    #167983
    Purpose
    Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    TETv4 (TET1CD-XTEN80-dCas9)
    Use
    CRISPR and Synthetic Biology
    Tags
    3xNLS, TET1 catalytic domain, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167983-plasmid-map-sequence-id-418937

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  34. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  36. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170348-plasmid-map-sequence-id-336137

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  40. dCas9-CBP core

    Plasmid
    #179543
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179543-plasmid-map-sequence-id-354769

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  41. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  42. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  43. p426_Cas9_gRNA-R-RDS1a

    Plasmid
    #87405
    Purpose
    p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87405-plasmid-map-sequence-id-193448

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  49. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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Showing: 7551 - 7600 of 12238 results