We narrowed to 17,089 results for: EMB
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Plasmid#179901PurposeDonor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianPromoterTRE3GSAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-07-mNeonGreen-R11a
Plasmid#179890PurposeDonor with homologous arms for Rab11a to knock in mNeonGreenDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mEGFP
Plasmid#105794PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mEGFPExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3/hArf5(WT)-EGFP
Plasmid#79534PurposeExpresses C-terminally EGFP-tagged Arf5(WT) in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP(S558A)-shTACC3
Plasmid#59357PurposeExpresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M
Plasmid#114181PurposeORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorAvailable SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorAvailable SinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-NGFR:LMP1-IRES-mRFP
Plasmid#36974DepositorUseRetroviralExpressionMammalianMutationNGFR:LMP1 chimeric receptor consists of aa 1–276 …PromoterCMVAvailable SinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH744-CEN-RLuc/slowmaxCFLuc
Plasmid#38224DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH742-CEN-RLuc/slowminCFLuc
Plasmid#38222DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-DIO-Lck-mCherry-ibARK(D110A)-4x6T
Plasmid#241244PurposeNegative control vrial expression vector for Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytesDepositorInsertDIO-Lck-mCherry-ibARK(D110A)-4x6T
UseAAVMutationD110AAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-DV-IRES-myr-mKate (JDW 494)
Plasmid#242575PurposeGateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.DepositorInsertattR1-CmR-ccdB-attR2
ExpressionMammalianPromoterCAGGSAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-RspA-NFAST
Plasmid#233595PurposeExpression of RspA-NFAST on the ER membraneDepositorInsertRspA-NFAST
ExpressionMammalianPromoterCMVAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-long-RspA-NFAST
Plasmid#233600PurposeExpression of RspA-NFAST on the ER membrane after a 55 aminoacid linkerDepositorInsertRspA-NFAST
ExpressionMammalianPromoterCMVAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX4
Plasmid#223340PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)
TagsEGFP-miniTurboID-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX3
Plasmid#223339PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)
TagsminiTurboID-EGFP-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only