We narrowed to 5,107 results for: PEP

1. pDONR223-NPR2

   Plasmid

   #23479

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NPR2 (NPR2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   isoform missing aa905-962

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAP-COMP-blac-flag-his

   Plasmid

   #111001

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   aminopeptidase P (APP) (PF14_0517 Synthetic)
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_LAP3_WT_V5

   Plasmid

   #82958

   Purpose

   Gateway Donor vector containing LAP3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   LAP3 (LAP3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAav-MDM2-PQS2-3xHA

   Plasmid

   #84886

   Purpose

   rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Inserts

   MDM2 genomic sequence HR1 (MDM2 Human)
   MDM2 genomic insert HR2 (MDM2 Human)
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Expression

   Mutation

   Promoter

   no

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-NPY [L115/13R]

   Plasmid

   #177461

   Purpose

   Mammalian Expression Plasmid of anti-NPY/Neuropeptide Y (Human). Derived from hybridoma L115/13.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal antibody (NPY Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExp-His-zBasic-RBD

   Plasmid

   #194999

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli

   Depositor

   Marko Hyvönen

   Article

   Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   His8-Zbasic

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-NPY2R [L119/106-rat IgG2a] - Chimeric

   Plasmid

   #227067

   Purpose

   Mammalian expression plasmid of anti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L119/106.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant mouse monoclonal antibody. (NPY2R Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CYP19B-COMP-blac-flag-his

   Plasmid

   #111007

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   peptidyl-prolyl cis-trans isomerase (CYP19B) (CyP22 Synthetic)
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Anti-NPY [L115/13-rat IgG2a] - Chimeric

   Plasmid

   #227066

   Purpose

   Mammalian expression plasmid of anti-NPY/Neuropeptide Y (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L115/13.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-NPY/Neuropeptide Y (Homo sapiens) recombinant mouse monoclonal antibody. (NPY Chimera: M. musculus (mouse)/R. norvegicus (rat))
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AIMTOR T757

    Plasmid

    #140828

    Purpose

    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR T757 (ULK1 Human)
    

    Use

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgLAP1

    Plasmid

    #19702

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Use

    Tags

    EGFP, S peptide, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgLAP2

    Plasmid

    #19703

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Use

    Tags

    Flag, S peptide, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pgLAP4

    Plasmid

    #19705

    Depositor

    Peter Jackson

    Insert

    pgLAP4
    

    Use

    Tags

    Flag, S peptide, and Tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pgLAP3

    Plasmid

    #19704

    Depositor

    Peter Jackson

    Insert

    pgLAP3
    

    Use

    Tags

    EGFP, S peptide, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pgLAP5

    Plasmid

    #19706

    Depositor

    Peter Jackson

    Insert

    pgLAP5
    

    Use

    Tags

    EGFP, Precission Protease site, and S peptide

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FtsZ

    Plasmid

    #74128

    Purpose

    Expresses FtsZ without a peptide

    Depositor

    Lynne Regan

    Insert

    FtsZ
    

    Use

    Tags

    C-terminal linker sequence (G G S G S G S S)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET283xFlagPP7CPHis

    Plasmid

    #28174

    Depositor

    Kathleen Collins

    Insert

    PP7 coat protein optimized
    

    Use

    Tags

    6xhistidine and triple FLAG peptide

    Expression

    Bacterial

    Mutation

    C68A,C73A

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-YFP-Bak-ER

    Plasmid

    #191948

    Purpose

    Expression of a YFP- Bak chimera and endoplasmatic reticulum signal peptide.

    Depositor

    Luca Scorrano

    Insert

    YFP-Bak-ER
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHN767

    Plasmid

    #178884

    Purpose

    Expression of bacterial PNGase

    Depositor

    Kazuhiro Abe

    Insert

    Peptide:N-glycosidase F
    

    Use

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRT7 NT Topo arabinose

    Plasmid

    #73446

    Purpose

    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

    Depositor

    Jesse Rinehart

    Type

    Empty backbone

    Use

    Tags

    GST (N-terminal), 6xHis (C-terminal)

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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